Dataset Information

Malonylation of Acetyl-CoA Carboxylase 1 Promotes Hepatic Steatosis and Is Attenuated by Ketogenic Diet in NAFLD

ABSTRACT: Protein post-translational modifications (PTMs) participate in important bioactive regulatory processes and therefore can help elucidate the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Here, we investigate the involvement of PTMs in ketogenic diet (KD)-improved fatty liver by multi-omics and reveal a core target of lysine malonylation, acetyl-CoA carboxylase 1 (ACC1). ACC1 protein levels and Lys1523 malonylation are significantly decreased by KD. It is discovered that a malonylation-mimic mutant in ACC1 increases its enzyme activity and stability to promote hepatic steatosis, whereas the malonylation-null mutant upregulates the ubiquitination degradation of ACC1. A customized Lys1523ACC1 malonylation antibody confirms the increased malonylation of ACC1 in the NAFLD samples. Overall, the lysine malonylation of ACC1 is attenuated by KD in NAFLD and plays an important role in promoting hepatic steatosis. Malonylation is critical for ACC1 activity and stability, highlighting the anti-malonylation effect of ACC1 as a potential strategy for treating NAFLD.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Hancheng Qin

LAB HEAD: Fen Xu

PROVIDER: PXD040481 | Pride | 2023-07-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	C9084LPMa_HFD_1_Slot2-24_1_4426.zip	Other
	C9084LPMa_HFD_3_Slot2-25_1_4428.zip	Other
	C9084LPMa_HFD_CD2_Slot2-28_1_4434.zip	Other
	C9084LPMa_HFD_CD3_Slot2-29_1_4436.zip	Other
	C9084LPMa_HFD_KD2_Slot2-26_1_4430.zip	Other

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Publications

Malonylation of Acetyl-CoA carboxylase 1 promotes hepatic steatosis and is attenuated by ketogenic diet in NAFLD.

Cao Huanyi H Cai Qingxian Q Guo Wanrong W Su Qiao Q Qin Hancheng H Wang Tian T Xian Yingxin Y Zeng Longyi L Cai Mengyin M Guan Haixia H Chen Sifan S Liang Hua H Xu Fen F

Cell reports 20230331 4

Protein post-translational modifications (PTMs) participate in important bioactive regulatory processes and therefore can help elucidate the pathogenesis of non-alcoholic fatty liver disease (NAFLD). Here, we investigate the involvement of PTMs in ketogenic diet (KD)-improved fatty liver by multi-omics and reveal a core target of lysine malonylation, acetyl-coenzyme A (CoA) carboxylase 1 (ACC1). ACC1 protein levels and Lys1523 malonylation are significantly decreased by KD. A malonylation-mimic ...[more]

PMID: 37002924

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