Dataset Information

Quantitative phosphoproteomics reveal novel roles of cAMP in plants

ABSTRACT: 3′,5′-cyclic adenosine monophosphate (cAMP) is finally recognized as an essential signaling molecule in plants where cAMP-dependent processes include responses to hormones and environmental stimuli. To better understand the role of cAMP at the systems level, we have undertaken a phosphoproteomic analysis to elucidate the cAMP-dependent response of tobacco BY-2 cells. These cells overexpress a “sponge” that buffers intracellular cAMP level. The results show that, firstly, in vivo cAMP dampening profoundly affects the plant kinome and notably mitogen-activated protein kinases, receptor-like kinases and calcium-dependent protein kinases, thereby modulating the cellular responses at the systems level. Secondly, buffering cAMP levels also affects mRNA processing through the modulation of the phosphorylation status of several RNA-binding proteins with roles in splicing, including many SR proteins. Thirdly, cAMP-dependent phosphorylation targets appear to be conserved among plant species. Taken together, these findings are consistent with an ancient role of cAMP in mRNA processing and cellular programming and suggest that unperturbed cellular cAMP levels are essential for cellular homeostasis and signaling in plant cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Nicotiana Tabacum (common Tobacco)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Milena Marsoni

LAB HEAD: Guido Domingo

PROVIDER: PXD040912 | Pride | 2024-05-24

REPOSITORIES: Pride

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The cAMP-dependent phosphorylation footprint in response to heat stress.

Domingo Guido G Marsoni Milena M Davide Eleonora E Fortunato Stefania S de Pinto Maria Concetta MC Bracale Marcella M Molla Gianluca G Gehring Chris C Vannini Candida C

Plant cell reports 20240507 6

<h4>Key message</h4>cAMP modulates the phosphorylation status of highly conserved phosphosites in RNA-binding proteins crucial for mRNA metabolism and reprogramming in response to heat stress. In plants, 3',5'-cyclic adenosine monophosphate (3',5'-cAMP) is a second messenger that modulates multiple cellular targets, thereby participating in plant developmental and adaptive processes. Although its role in ameliorating heat-related damage has been demonstrated, mechanisms that govern cAMP-dependen ...[more]

PMID: 38713285

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			Action	DRS
	CAS_27_P_1.raw	Raw
	CAS_27_P_2.raw	Raw
	CAS_27_P_3.raw	Raw
	CAS_27_P_4.raw	Raw
	SearchPhospho.txt	Txt