Dataset Information

VRK1 regulates sensitivity to oxidative stress by altering the nuclear phosphoproteome and histone epigenetic modifications

ABSTRACT: Chromatin organization and its dynamic remodeling determine its accessibility and sensitivity to DNA damage. The major source of endogenous DNA damage is oxidative stress. We studied the role of the VRK1 chromatin kinase in the response to oxidative stress, which alters the nuclear phosphoproteome and the histone epigenetic pattern. Oxidative stress causes an accumulation of 8-oxoG DNA lesions that were significantly increased by VRK1 depletion, causing a significant accumulation of DNA strand breaks detected by their labeling with TUNEL assays. The analysis of the nuclear phosphoproteome in response to oxidative stress detected an enrichment of phosphorylated proteins associated with chromosome organization and chromatin remodeling, and these protein phosphorylations significantly decreased after VRK1 depletion. Because chromatin remodeling requires covalent modifications in the histone tails, we studied the effect of oxidative stress on the pattern of histones H4 and H3 epigenetic modifications. Oxidative stress induced the acetylation of H4 in K16, an increase of H3K9 acetylation, and a loss of H3K4me3. Depletion of VRK1 altered all these modifications induced by oxidative stress and resulted in the loss of H4K16ac and H3K9ac and an increase in H3K9me3 and H3K4me3 levels. All these changes induced by oxidative stress in the epigenetic pattern of histones are impaired by the depletion of VRK1, indicating that VRK1 plays a major role in the functional reorganization of chromatin in the response to oxidative stress. VRK1 depletion alters the nuclear phosphoproteome and the histone epigenetic pattern in response to oxidative stress.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Thang Pham

LAB HEAD: Connie Jimenez

PROVIDER: PXD040944 | Pride | 2024-05-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	MaxQuant-txt-IMAC.zip	Other
	MaxQuant-txt-lysate.zip	Other
	QE3_220308_OPL0000_EN_VRK1_nucl_fr_IMAC_A.raw	Raw
	QE3_220308_OPL0000_EN_VRK1_nucl_fr_IMAC_AA.raw	Raw
	QE3_220308_OPL0000_EN_VRK1_nucl_fr_IMAC_AB.raw	Raw

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Publications

VRK1 Regulates Sensitivity to Oxidative Stress by Altering Histone Epigenetic Modifications and the Nuclear Phosphoproteome in Tumor Cells.

Navarro-Carrasco Elena E Monte-Serrano Eva E Campos-Díaz Aurora A Rolfs Frank F de Goeij-de Haas Richard R Pham Thang V TV Piersma Sander R SR González-Alonso Paula P Jiménez Connie R CR Lazo Pedro A PA

International journal of molecular sciences 20240430 9

The chromatin organization and its dynamic remodeling determine its accessibility and sensitivity to DNA damage oxidative stress, the main source of endogenous DNA damage. We studied the role of the VRK1 chromatin kinase in the response to oxidative stress. which alters the nuclear pattern of histone epigenetic modifications and phosphoproteome pathways. The early effect of oxidative stress on chromatin was studied by determining the levels of 8-oxoG lesions and the alteration of the epigenetic ...[more]

PMID: 38732093

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