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LiP-MS on HEK293 lysate treated with Probe Candidate TR05267290 and Negative Control TR05706192

ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated HEK293 cell lysate with the candidate probe TR05267290 and the corresponding negative control TR05706192 to profile target engagement and other structural effects. This project is part of the IMI EUbOPEN project and was performed in collaboration with Kilian Huber (University of Oxford).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Viviane Reber

LAB HEAD: Dr. Matthias Gstaiger

PROVIDER: PXD041098 | Pride | 2023-09-01

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200320_human_PK_iRT.fasta	Fasta
	20230322_214930_VR_Ex31_probe1_Report.xls	Xls
	230224_LU1_reberv_Ex31_1.raw	Raw
	230224_LU1_reberv_Ex31_10.raw	Raw
	230224_LU1_reberv_Ex31_11.raw	Raw

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