Proteomics

Dataset Information

0

LiP-MS on HEK293 lysate treated with Probe Candidate TR05481366 and Negative Control TR05302201


ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated HEK293 cell lysate with the candidate probe TR05481366 and the corresponding negative control TR05302201 to profile target engagement and other structural effects. This project is part of the IMI EUbOPEN project and was performed in collaboration with Kilian Huber (University of Oxford).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Viviane Reber  

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD041100 | Pride | 2023-09-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
200320_human_PK_iRT.fasta Fasta
20230322_214959_VR_Ex31_probe3_Report.xls Xls
230224_LU1_reberv_Ex31_1.raw Raw
230224_LU1_reberv_Ex31_25.raw Raw
230224_LU1_reberv_Ex31_26.raw Raw
Items per page:
1 - 5 of 29

Similar Datasets

2023-04-26 | PXD041099 | Pride
2023-09-01 | PXD041098 | Pride
2023-04-26 | PXD041089 | Pride
2023-04-26 | PXD035183 | Pride
2018-06-06 | PXD009714 | Pride
2023-04-03 | GSE228809 | GEO
2024-02-10 | PXD039481 | Pride
2023-04-20 | PXD041544 | Pride
2021-12-31 | GSE162874 | GEO
2021-12-31 | GSE162877 | GEO