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LiP-MS on HEK293 lysate treated with rapamycin

ABSTRACT: Limited proteolysis coupled to mass spectrometry (LiP-MS) is a structural proteomics technique that can be used to identify the targets of small molecules. This is achieved by incubating lysate with the drug of interest, which induces structural changes such as occupation of the binding site or changes and protein-protein interactions. This is followed by a limited proteolysis step in which the unspecific protease proteinase K is added to the treated lysate. The protease preferentially cleaves accessible and flexible regions of the proteins, thus generating peptides that reflect the changes in surface accessibility caused by the treatment with the small molecule. Here we treated HEK293 cell lysate with rapamycin and identify the known main target FKBP1A. This data is intended to be used as a model dataset for LiP-MS data evaluation and was created as part of the IMI EUbOPEN project.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Early Embryonic Cell

SUBMITTER: Viviane Reber

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD035183 | Pride | 2023-04-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	190417_Human_sp_only_iRT.fasta	Fasta
	200707_EX1_VR_Ex01_DMSO_LiP_1_3ul.raw	Raw
	200707_EX1_VR_Ex01_DMSO_LiP_2.raw	Raw
	200707_EX1_VR_Ex01_DMSO_LiP_3.raw	Raw
	200707_EX1_VR_Ex01_DMSO_LiP_4.raw	Raw

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