Dataset Information

Growth rate and limiting substrate define the nutritional composition and cell size of microbial biomass for food applications

ABSTRACT: Microbial biomass has emerged as a promising sustainable protein alternative. The cultivation conditions affect the composition of microbial cells, and hence their quality and nutritional value. Here, we investigated the relationship between growth rate, substrate availability and cell composition and size of Cupriavidus necator and Komagataella phaffii. Biomass with decreased nucleic acid and increased protein content was produced at low growth rates. Conversely, high rates resulted in larger cells, which could enable more efficient biomass harvesting. The proteome allocation varied across the different growth rates, with more ribosomal proteins at higher rates. Considering the distinct amino acid profiles established for the different cellular components, variations in their abundance impacts the product quality leading to higher cysteine and phenylalanine content at low growth rates. In summary, we demonstrate tradeoffs between nutritional quality and production rate, and we discuss that techno-functional food properties should be considered when designing microbial biomass production processes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Cupriavidus Necator Komagataella Phaffii

SUBMITTER: Robbin Bouwmeester

LAB HEAD: Korneel Rabaey

PROVIDER: PXD041491 | Pride | 2024-01-26

REPOSITORIES: pride

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			Action	DRS
	B101860_Bp_uPac18_trap2_PRC-5613_Rabaey_Sakarika_shotgun_1_20220404171609.raw	Raw
	B101862_Bp_uPac18_trap2_PRC-5613_Rabaey_Sakarika_shotgun_2.raw	Raw
	B101864_Bp_uPac18_trap2_PRC-5613_Rabaey_Sakarika_shotgun_3.raw	Raw
	B101866_Bp_uPac18_trap2_PRC-5613_Rabaey_Sakarika_shotgun_4.raw	Raw
	B101868_Bp_uPac18_trap2_PRC-5613_Rabaey_Sakarika_shotgun_5.raw	Raw

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The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions.

Sakarika Myrsini M Kerckhof Frederiek-Maarten FM Van Peteghem Lotte L Pereira Alexandra A Van Den Bossche Tim T Bouwmeester Robbin R Gabriels Ralf R Van Haver Delphi D Ulčar Barbara B Martens Lennart L Impens Francis F Boon Nico N Ganigué Ramon R Rabaey Korneel K

Microbial cell factories 20231211 1

<h4>Background</h4>It is increasingly recognized that conventional food production systems are not able to meet the globally increasing protein needs, resulting in overexploitation and depletion of resources, and environmental degradation. In this context, microbial biomass has emerged as a promising sustainable protein alternative. Nevertheless, often no consideration is given on the fact that the cultivation conditions affect the composition of microbial cells, and hence their quality and nutr ...[more]

PMID: 38072930

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