Dataset Information

Identification of co-factors of eIF4E1b protein in mouse ovary.

ABSTRACT: We have identified eukaryotic translation initiation factor 4E family member 1B (Eif4e1b) as a regulator of maternal RNA translation, whose maternal deletion leads to 2-cell arrest phenotype in mouse embryos. eIF4E1b protein binds mRNA selectively in oocytes and controls their translation in early embryos. eIF4E1b expresses specifically in mouse ovary and we have generated a knock-in mouse line in which HA tag is conjugated to the C terminus of eIF4E1b. We aim to identify eIF4E1b co-factors using mass spectrometry after anit-HA immunoprecipitation (IP) using mouse ovaries.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Ovary

SUBMITTER: David Anderson

LAB HEAD: Jurrien Dean

PROVIDER: PXD041622 | Pride | 2023-06-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	allSpectra.CID.ITMS.secpep.sil0_0.apl	Other
	allSpectra.CID.ITMS.secpep.sil0_1.apl	Other
	allSpectra.CID.ITMS.secpep.sil0_10.apl	Other
	allSpectra.CID.ITMS.secpep.sil0_11.apl	Other
	allSpectra.CID.ITMS.secpep.sil0_12.apl	Other

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Publications

Germ cell-specific eIF4E1b regulates maternal mRNA translation to ensure zygotic genome activation.

Yang Guanghui G Xin Qiliang Q Feng Iris I Wu Di D Dean Jurrien J

Genes & development 20230531 9-10

Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remain unknown. Using gene-edited mice, we document that the oocyte-specific eukaryotic translation initiation factor 4E family member 1b (eIF4E1b) is the regulator of maternal mRNA expression that ensures subsequent repro ...[more]

PMID: 37257918

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