Dataset Information

Distinct lactation-associated macrophages exist in murine mammary tissue and human milk

ABSTRACT: Macrophages are involved in immune defense, organogenesis and tissue homeostasis. They also contribute to the different phases of mammary gland remodeling during development, pregnancy and involution post-lactation. Yet, less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multi-parameter flow cytometry and single-cell RNA sequencing we reveal this population as distinct from the two resident macrophage subsets present pregestationally. These lactation-induced macrophages (LiMacs) are predominantly monocyte-derived and expand by proliferation in situ concomitant with nursing. LiMacs develop independently of IL-34 but require CSF-1 signaling and are partly microbiota-dependent. Locally, they reside adjacent to the basal cells of the alveoli and extravasate into the milk. Moreover, we also found several macrophage subsets in human milk, resembling LiMacs. Collectively, these findings reveal the emergence of unique macrophages in the mammary gland and milk during lactation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Milk

SUBMITTER: Ekaterina Petrova

LAB HEAD: Melanie Greter

PROVIDER: PXD041711 | Pride | 2023-06-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20200728_006_S220954_WT_3_Wild_Type_WT.raw	Raw
	20200728_008_S220953_WT_2_Wild_Type_WT.raw	Raw
	20200728_010_S220952_WT_1_Wild_Type_WT.raw	Raw
	20200728_012_S220951_KO_3_Knock_out_KO.raw	Raw
	20200728_014_S220950_KO_2_Knock_out_KO.raw	Raw

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Publications

Lactation-associated macrophages exist in murine mammary tissue and human milk.

Cansever Dilay D Petrova Ekaterina E Krishnarajah Sinduya S Mussak Caroline C Welsh Christina A CA Mildenberger Wiebke W Mulder Kevin K Kreiner Victor V Roussel Elsa E Stifter Sebastian A SA Andreadou Myrto M Zwicky Pascale P Jurado Nicole Puertas NP Rehrauer Hubert H Tan Ge G Liu Zhaoyuan Z Blériot Camille C Ronchi Francesca F Macpherson Andrew J AJ Ginhoux Florent F Natalucci Giancarlo G Becher Burkhard B Greter Melanie M

Nature immunology 20230619 7

Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlactation. Less is known about the dynamics of mammary gland macrophages in the lactation stage. Here, we describe a macrophage population present during lactation in mice. By multiparameter flow cytometry and single-cell RNA sequencing, we identified a lactation-induced CD11c<sup>+</sup>CX3CR ...[more]

PMID: 37337103

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