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N-glycoproteomics and SESTAR++ analysis based on SeMOE

ABSTRACT: The isotopic envelope pattern of selenium is guaranteed by its six stable isotopes with distinctive distribution (74Se, 0.89%; 76Se, 9.37%; 77Se, 7.63%; 78Se, 23.77%; 80Se, 49.61%; 82Se, 8.73%). We postulated that the effective incorporation of selenosugar in glycoproteins would achieve direct isotopic pattern prediction for glycopeptides, without the need for secondary tagging procedures. Consequently, we performed comparative studies using a previously reported computational algorithm, the updated version of selenium-encoded isotopic signature targeted profiling (SESTAR++)31, 32, and a glycan-first glycopeptide search engine, pGlyco3 to comprehensively analyze the intact N-glycopeptides in cells treated with SeMOE probes.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Xiao Tian

LAB HEAD: Ran Xie

PROVIDER: PXD042137 | Pride | 2023-11-20

REPOSITORIES: Pride

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			Action	DRS
	Lumos3_2ul_HeLa_control_150um_30cm_150min_HCD_gradient_3_20210719_F1_R1.raw	Raw
	Lumos3_2ul_TX_Hela_9AZ_150um_30cm_150min_HCD_OT_OT_20210809_F1_R1.raw	Raw
	Lumos3_2ul_TX_Hela_AM_150um_30cm_150min_HCD_OT_OT_20210809_F1_R1.raw	Raw
	Lumos3_2ul_TX_Hela_M_150um_30cm_150min_HCD_OT_OT_20210809_F1_R1.raw	Raw
	Lumos3_2ul_TX_Hela_PM_150um_30cm_150min_HCD_OT_OT_20210809_F1_R1.raw	Raw

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