Proteomics

Dataset Information

0

Development and systematic evaluation of decellularization protocols for rat diaphragms

ABSTRACT: We decellularized rat diaphragms with the main detergents 1% or 0.1% sodium dodecyl sulfate (SDS) and 4% sodium deoxycholate (SDC) by orbital shaking (OS) or retrograde perfusion (RP) through the vena cava. Our systematic comparison entails evaluation of decellularized diaphragmatic samples by (1) quantitative analysis including DNA quantification and biomechanical testing, (2) qualitative and semi-quantitative analysis by proteomics, as well as (3) qualitative assessment with macroscopic and microscopic evaluation by histological staining, immunohistochemistry and scanning electron microscopy.

INSTRUMENT(S): impact II

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Diaphragm

SUBMITTER: Karl Hillebrandt  

LAB HEAD: Karl Herbert Hillebrandt

PROVIDER: PXD042219 | Pride | 2024-05-07

REPOSITORIES: pride

Json Xml

Dataset's files

Source:
Action DRS
KH-Decell28-112021-10_RB6_01_5579.d.zip Other
KH-Decell28-112021-10_RB6_01_5579.mgf Mgf
KH-Decell28-112021-10_RB6_01_5579_4.4.200.xml Xml
KH-Decell28-112021-11_RC5_01_5586.d.zip Other
KH-Decell28-112021-11_RC5_01_5586.mgf Mgf
Items per page:
1 - 5 of 85
altmetric image

Publications

Development and systematic evaluation of decellularization protocols in different application models for diaphragmatic tissue engineering.

Andreas Marco N MN   Boehm Agnes K AK   Tang Peter P   Moosburner Simon S   Klein Oliver O   Daneshgar Assal A   Gaßner Joseph M G V JMGV   Raschzok Nathanael N   Haderer Luna L   Wulsten Dag D   Rückert Jens-Carsten JC   Spuler Simone S   Pratschke Johann J   Sauer Igor M IM   Hillebrandt Karl H KH  

Biomaterials advances 20230605

<h4>Background</h4>Tissue engineered bioscaffolds based on decellularized composites have gained increasing interest for treatment of various diaphragmatic impairments, including muscular atrophies and diaphragmatic hernias. Detergent-enzymatic treatment (DET) constitutes a standard strategy for diaphragmatic decellularization. However, there is scarce data on comparing DET protocols with different substances in distinct application models in their ability to maximize cellular removal while mini  ...[more]

Similar Datasets

Proteomics Protein Expression in Liver Tissue and Bile of Elderly Sprague-Dawley Rats after Normothermic Machine Perfusion
2024-08-09 | PXD045394 | Pride
Proteomics Environmental Enrichment and Rat Liver Proteomics
2024-01-22 | PXD046110 | Pride
Proteomics decellularized hepatic and renal tissue
2024-05-07 | PXD049189 | Pride
Proteomics Porcine brain tissue-derived decellularized extracellular matrix LC-MS/MS
2023-11-24 | PXD046191 | Pride
Proteomics Porcine pancreas tissue-derived decellularized extracellular matrix LC-MS/MS
2025-01-27 | PXD047892 | Pride
Proteomics Proteomic analysi of decellularized porcine pancreas
2020-03-23 | PXD014356 | Pride
Proteomics Xenografted Tumor decellularization provides cell-specific in vitro 3D environment
2022-09-12 | PXD034597 | Pride
Unknown E-PROT-63
2022-01-10 | E-PROT-63 | ExpressionAtlas
Transcriptomics mRNA-seq of mouse lung vascular endothelial cells and mouse liver sinusoidal endothelial cells before and after their partial reprogramming into progenitor-like cells and recellularization of decellularized mouse lung scaffolds
2020-06-23 | E-MTAB-8394 | biostudies-arrayexpress
Proteomics Extracellular matrix educates a tumor macrophage phenotype found in ovarian cancer metastasis
2023-04-11 | PXD036940 | Pride