Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of the original binder and modern contaminants in distemper paints from Uvdal stave church, Norway

ABSTRACT: Two distemper paint samples taken from decorative boards in Uvdal church, Norway, were analysed using palaeoproteomics, with an aim of identifying their binder and possible contaminants. The results point at the use of calfskin to produce hide glue as the original binder. These results are consistent with the instructions of binder production and resource allocation in the historical records of Norway. Additionally, there was no evidence of contamination with sturgeon glue from 1990’s restoration treatments. However, we found other ‘contaminants’ including a large quantity of human saliva proteins along with a few proteins corresponding to oats and barley, likely transferred with the saliva and likely occurring from the boards’ discovery in the 1970s. This work illustrates the need to fully consider contamination sources in palaeoproteomics and to inform those working with such objects about the potential for their contamination.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Skin, Bone, Saliva

SUBMITTER: Zahra Haghighi

LAB HEAD: Enrico Cappellini

PROVIDER: PXD043606 | Pride | 2024-06-05

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_labblank_2008.raw	Raw
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_paint_2009.raw	Raw
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_paint_2010.raw	Raw
	txt.zip	Other
	uniprot-download_true_format_fasta_query_accession_3AA1ZAB5_20OR_20a-2022.09.26-13.39.37.63.fasta	Fasta

Items per page:

1 - 5 of 5

Similar Datasets

Palmyrene Polychromy: Investigations of funerary portraits from Palmyra in the collections of the Ny Carlsberg Glyptotek

Project description:Two samples from the Beauty of Palmyra (IN 2795 – Ny Carlsberg Glyptotek, Copenhagen, Denmark) were collected from the surface coating, and partially the paint layer, in locations corresponding to paint residues from respectively the yellow paint (containing lead-based yellow pigment) and the red paint (containing haematite as pigment) of the headdress of the figure. The two samples were analysed using a palaeoproteomic protocol to investigate the use of a proteinaceous paint binder and/or the presence of other proteinaceous materials. The analysis led to the confident identification of collagen, probably indicating the use of animal glue as paint binder of the sample removed from red paint residues. Various animal keratins were also confidently identified in both samples. The presence of these proteins might be related to the manufacture of organic pigments, but also possibly to the use of a keratin-based glue as paint binder or surface coating, or even to the use of a paintbrush made with animal bristles.

2022-06-28 | PXD031280 | Pride

Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus

Project description:Mineralised dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. In this study we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of 16th century human dental calculus from an individual from Trondheim, Norway. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. M. leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual’s dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record.

2020-10-19 | PXD017836 | Pride

The CDK12-inhibitor CR8 mediates Cyclin K degradation through the CUL4-RBX1-DDB1 E3 ubiquitin ligase

Project description:Small molecules have been identified that induce protein-protein interactions between a substrate and a ubiquitin ligase, resulting in targeted protein degradation. Such molecular glue compounds, unlike traditional enzyme inhibitors, act sub-stoichiometrically to catalyse rapid depletion of previously inaccessible targets. Molecular glue degraders have the potential for clinical efficacy, but have thus far only been discovered serendipitously. Through correlations between compound cytotoxicity and E3 ligase expression levels, we found that CR8, a cyclin-dependent kinase (CDK) inhibitor, acts as a molecular glue degrader. A surface-exposed pyridyl moiety of CR8 is sufficient to induce complex formation between CDK12-Cyclin K and DDB1, a component of CUL4 E3 ubiquitin ligase complexes, which presents Cyclin K for ubiquitination and degradation. Our results reveal that minor surface-exposed modifications confer gain-of-function glue properties to an inhibitor. We hypothesize that chemical alteration of surface-exposed moieties is a broad strategy to turn a target binder into a molecular glue.

2020-06-10 | PXD016188 | Pride

The CDK12-inhibitor CR8 mediates Cyclin K degradation through the CUL4-RBX1-DDB1 E3 ubiquitin ligase

2020-06-10 | PXD016187 | Pride

Use of hide glue in 4th millennium BC Europe

Project description:To test whether hide or fish glue was used to fix bark decorations to the body of a wood bow (of a different species) found during the archaeological excavation of at the site “Parkhaus-Opéra” in Zurich (Switzerland), dated by dendrochronology to 3175 - 3157 BC, we attempted extraction of collagen residues from wood flakes collected from two areas of the bow surface under the bark and their identification by on-line nanoflow reversed-phase C18 liquid chromatography tandem mass spectrometry.

2015-08-20 | PXD001727 | Pride

Molecular characterization of adhesives (glue lining pastes) used in restoration

Project description:The molecular characterization of samples from works of art can provide valuable insights into the composition of ancient restoration materials and their conservation state. Here, we present a novel experimental protocol for the molecular characterization of a specific adhesive used in historical painting restoration, known as "glue lining pastes." Due to the high molecular complexity of these adhesives, we propose a multi-step extraction protocol to simultaneously recover and fractionate from a single microsample the three main classes of biomolecules contained in glue pastes (lipids, polysaccharides, and proteins). High-performance separation coupled with high-resolution MS techniques were applied to the isolated fractions to identify specific components. The proposed method was optimized using test specimens of various traditional glue pastes applied to canvases and successfully applied to a historical glue paste sample from the 17th-century painting "La fuga in Egitto," part of the Pagliara collection at the University Suor Orsola Benincasa (Naples, Italy). The data collected in this work provide insights into the specific recipe used for adhesive preparation, supporting artistic and historical interpretations, and contributing to a broader understanding of old restoration practices.

2024-10-17 | PXD051480 | Pride

Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

Project description:Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and mallard egg white. Analysis of posttranslational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV-light and was likely applied long before the addition of the glue. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.

2018-04-05 | PXD008486 | Pride

Modern Paint Binder Controls for Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting

2018-04-05 | PXD009298 | Pride

Palaeoproteomics guidelines to identify proteinaceous binders in artworks following the study of a 15th‑century painting by Sandro Botticelli’s workshop

Project description:Undertaking the conservation of artworks informed by the results of molecular analyses has gained growing importance over the last decades, and today it can take advantage of state-of-the-art analytical techniques, such as mass spectrometry-based proteomics. Protein-based binders are among the most common organic materials used in artworks, having been used in their production for centuries. However, the applications of proteomics to these materials are still limited. In this work, a palaeoproteomic workflow was successfully tested on paint reconstructions, and subsequently applied to micro-samples from a 15th-century panel painting, attributed to the workshop of Sandro Botticelli. This method allowed the confident identification of the protein-based binders and their biological origin, as well as the discrimination of the binder used in the ground and paint layers of the painting. These results show that the approach is accurate, highly sensitive, and broadly applicable in the cultural heritage field, due to the limited amount of starting material required. Accordingly, a set of guidelines are suggested, covering the main steps of the data analysis and interpretation of protein sequencing results, optimised for artworks.

2022-07-05 | PXD024397 | Pride

Vittrup Man – the life-history of a genetic foreigner in Neolithic Denmark

Project description:The deadly maltreated body of Vittrup Man was deposited in a Danish bog, probably as part of a ritualised sacrifice. It happened between c. 3300 and 3100 cal years BC, i.e., during the period of the local farming-based Funnel Beaker Culture. In terms of skull morphological features he differs from the majority of the contemporaneous farmers found in Denmark, and associates with hunter-gatherers, who inhabited Scandinavia during the previous millennia. His skeletal remains were selected for transdisciplinary analysis to reveal his life-history in terms of a population historical perspective. We report the combined results of an integrated set of genetic, isotopic, physical anthropological and archaeological analytical approaches. Strontium signature suggests a foreign birthplace that could be in Norway or Sweden. In addition, enamel oxygen isotope values indicate that as a child he lived in a colder climate, i.e., to the north of the regions inhabited by farmers. Genomic data in fact demonstrates that he is closely related to Mesolithic humans known from Norway and Sweden. Moreover, dietary stable isotope analyses on enamel and bone collagen demonstrate a fisher-hunter way of life in his childhood and a diet typical of farmers later on. Such a volatile life-history is also reflected by proteomic analysis of hardened organic deposits on his teeth, indicating the consumption of forager food (seal, whale and marine fish) as well as farmer food (sheep/goat). From a dietary isotopic transect of one of his teeth it is shown that his transfer between societies of foragers and farmers took place near to the end of his late teenage years.

2024-02-16 | PXD044743 | Pride