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Identification of the original binder and modern contaminants in distemper paints from Uvdal stave church, Norway

ABSTRACT: Two distemper paint samples taken from decorative boards in Uvdal church, Norway, were analysed using palaeoproteomics, with an aim of identifying their binder and possible contaminants. The results point at the use of calfskin to produce hide glue as the original binder. These results are consistent with the instructions of binder production and resource allocation in the historical records of Norway. Additionally, there was no evidence of contamination with sturgeon glue from 1990’s restoration treatments. However, we found other ‘contaminants’ including a large quantity of human saliva proteins along with a few proteins corresponding to oats and barley, likely transferred with the saliva and likely occurring from the boards’ discovery in the 1970s. This work illustrates the need to fully consider contamination sources in palaeoproteomics and to inform those working with such objects about the potential for their contamination.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Skin, Bone, Saliva

SUBMITTER: Zahra Haghighi

LAB HEAD: Enrico Cappellini

PROVIDER: PXD043606 | Pride | 2024-06-05

REPOSITORIES: Pride

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			Action	DRS
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_labblank_2008.raw	Raw
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_paint_2009.raw	Raw
	20211206_EXPL2_nLC3_MEM_collab_77min_DDA_Ancient_paint_2010.raw	Raw
	txt.zip	Other
	uniprot-download_true_format_fasta_query_accession_3AA1ZAB5_20OR_20a-2022.09.26-13.39.37.63.fasta	Fasta

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