Proteomics

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Multi-omic detection of Mycobacterium leprae in archaeological human dental calculus


ABSTRACT: Mineralised dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. In this study we present a Mycobacterium leprae genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of 16th century human dental calculus from an individual from Trondheim, Norway. Moreover, ancient mycobacterial peptides were retrieved via mass spectrometry-based proteomics, further validating the presence of the pathogen. M. leprae can readily be detected in the oral cavity and associated mucosal membranes, which likely contributed to it being incorporated into this individual’s dental calculus. This individual showed some possible, but not definitive, evidence of skeletal lesions associated with early stage leprosy. This study is the first known example of successful multi-omics retrieval of M. leprae from archaeological dental calculus. Furthermore, we offer new insights into dental calculus as an alternative sample source to bones or teeth for detecting and molecularly characterizing M. leprae in individuals from the archaeological record.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Dental Plaque

SUBMITTER: Anna Fotakis  

LAB HEAD: Thomas P. Gilbert

PROVIDER: PXD017836 | Pride | 2020-10-19

REPOSITORIES: Pride

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20160510_QE3_nLC5_RJC_COLLAB_GeoG_932_01.raw Raw
20160510_QE3_nLC5_RJC_COLLAB_GeoG_944_01.raw Raw
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Publications

Multi-omic detection of <i>Mycobacterium leprae</i> in archaeological human dental calculus.

Fotakis Anna K AK   Denham Sean D SD   Mackie Meaghan M   Orbegozo Miren Iraeta MI   Mylopotamitaki Dorothea D   Gopalakrishnan Shyam S   Sicheritz-Pontén Thomas T   Olsen Jesper V JV   Cappellini Enrico E   Zhang Guojie G   Christophersen Axel A   Gilbert M Thomas P MTP   Vågene Åshild J ÅJ  

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20201005 1812


Mineralized dental plaque (calculus) has proven to be an excellent source of ancient biomolecules. Here we present a <i>Mycobacterium leprae</i> genome (6.6-fold), the causative agent of leprosy, recovered via shotgun sequencing of sixteenth-century human dental calculus from an individual from Trondheim, Norway. When phylogenetically placed, this genome falls in branch 3I among the diversity of other contemporary ancient strains from Northern Europe. Moreover, ancient mycobacterial peptides wer  ...[more]

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