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Identify SUMO-modified proteins and determine the potential SUMO-specific protease target

ABSTRACT: Because SUMO modifications can change the molecular weight and/or the isoelectric point of target proteins, or cause degradation of target protein as well, we then compared the proteome of V592 and VdT-DNA using two-dimensional gel electrophoresis to separate proteins on the basis of their size and of their charge.

INSTRUMENT(S): 4700 Proteomics Analyzer

ORGANISM(S): Verticillium Dahliae Vdls.17

TISSUE(S): Fungus

SUBMITTER: bosen zhang

LAB HEAD: huishan guo

PROVIDER: PXD043986 | Pride | 2023-08-01

REPOSITORIES: Pride

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Matrisome remodelling during Intervertebral Disc development and ageing

Project description:Intervertebral disc (IVD) degeneration is often the cause of low back pain. Degeneration occurs with age and is accompanied by extracellular matrix (ECM) depletion, culminating in nucleus pulpous (NP) extrusion and IVD destruction. The changes that occur in the disc with age have been under investigation. However, a thorough study of ECM remodelling is needed, to better understand IVD development and age-associated degeneration. As so, iTRAQ LC-MS/MS analysis of foetus, young and old bovine NPs, was used to define the NP matrisome. The enrichment of Collagen XII and XIV in foetus, Fibronectin and Prolargin in elder samples and Collagen XI in young ones was independently validated. This study provides the first matrisome database of healthy discs during development and ageing, which is key to determine the pathways and processes that maintain disc homeostasis. The factors identified may help to explain age-associated IVD degeneration or constitute putative effectors for disc regeneration.

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Wheat Allergens MALDI-TOF/TOF - Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts

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Global proteomic analysis deciphers the mechanism of action of plant derived oleic acid against Candida albicans virulence and biofilm formation

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Proteomic analysis revealed the important role of vimentin in human cervical carcinoma HeLa cells treated with gambogic acid

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