Dataset Information

Quantitative differences in rumen epithelium proteins in lambs fed wheat, perennial wheat or perennial wheat plus lucerne.

ABSTRACT: The rumen is a major the site of digestion and absorption of energy predominantly in the form of short chain fatty acids as well as amino acids and simple carbohydrates. The value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. The research sought to determine if these diets representing high potassium (K): low sodium (Na) ratio (W, PW and PWL) was associated with changes in the membrane bound proteins that transport nutrients in the rumen epithelium (RE). Information about these proteins and the influence of different diets is scarce due to difficulties developing a procedure to examine them using quantitative proteomics. The research focuses on proteins extracted using an enzymatic procedure (Bond et al. 2019) from the rumen epithelium layer of the sheep’s’ forestomach. A membrane protein enriched fraction was then extracted and prepared for TMT-MS. The comprehensive assignment of proteins (>4000) to cellular location and quantification of differences in protein abundance will progress the annotation and understanding of protein functions in the ruminant genomes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Ovis Aries

TISSUE(S): Rumen Epithelium, Epithelial Cell

DISEASE(S): Disease Free

SUBMITTER: Karthik Shantharam kamath

LAB HEAD: Dr Jude Bond

PROVIDER: PXD044135 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	200706_P31234_TMT_Group_A_HFX_HpH_Fraction_06.raw	Raw
	200706_P31234_TMT_Group_B_HFX_HpH_Fraction_-1.raw	Raw
	200706_P31234_TMT_Group_B_HFX_HpH_Fraction_-2.raw	Raw
	200706_P31234_TMT_Group_B_HFX_HpH_Fraction_-3.raw	Raw
	200706_P31234_TMT_Group_B_HFX_HpH_Fraction_01.raw	Raw

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Publications

Quantitative Differences in Rumen Epithelium Proteins in Lambs Fed Wheat, Perennial Wheat, or Perennial Wheat plus Lucerne.

Bond Jude Jessie JJ Refshauge Gordon G Newell Matthew T MT Holman Benjamin W B BWB Wheeler David D Woodgate Serey S Kamath Karthik S KS Hayes Richard C RC

Proteomes 20230920 3

The value of crops such as perennial wheat (PW) for grain and grazing compared to conventional wheat (W), or the addition of lucerne to PW (PWL) is still being determined. This research sought to determine if these diets were associated with changes in the membranebound proteins that transport nutrients in the rumen epithelium (RE). Crossbred ewes (Poll Dorset × Merino) were fed W, PW, or PWL (50:50) fresh-cut forage <i>ad libitum</i> for 4 weeks. Average daily gain (ADG; <i>p</i> < 0.001) was h ...[more]

PMID: 37755706

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