Dataset Information

Myosin PTM peptide mapping from hibernating and active bears and squirrels

ABSTRACT: Recent discoveries in the field of skeletal muscle have found that the contractile protein myosin is able to dictate the resting metabolic rate of this organ. In this study we aimed to investigate if myosin is involved in the process of metabolic shutdown which is observed during hibernation. Comparing large and small hibernating mammals, we found that in small hibernating mammals, the rate of ATP turnover dictated by myosin was altered and that this was also dependent upon the temperature of skeletal muscle. We further investigated changes to the whole proteome of these animals and observed that in small hibernators found significant changes to sarcomere organization during hibernating periods. Finally, we identified hyperphosphorylation upon the myosin molecule in these small hibernators which was predicted to induce stability changes to this molecule. In this dataset, we performed PTM peptide mapping of the myosin protein derived from skeletal muscle fibre samples from squirrels collected in summer and winter time.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Ursus Arctos (brown Bear) (grizzly Bear) Spermophilus Tridecemlineatus (thirteen-lined Ground Squirrel)

TISSUE(S): Skeletal Muscle Fiber

SUBMITTER: Michael Wierer

LAB HEAD: Julien Ochala

PROVIDER: PXD044685 | Pride | 2024-04-29

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20230303_ASC1_NEO1_PRI_X670_P79_R654_60min_AUR2_25cm_59ms_1uL_S1.raw	Raw
	20230303_ASC1_NEO1_PRI_X670_P79_R654_60min_AUR2_25cm_59ms_1uL_S10.raw	Raw
	20230303_ASC1_NEO1_PRI_X670_P79_R654_60min_AUR2_25cm_59ms_1uL_S11.raw	Raw
	20230303_ASC1_NEO1_PRI_X670_P79_R654_60min_AUR2_25cm_59ms_1uL_S12.raw	Raw
	20230303_ASC1_NEO1_PRI_X670_P79_R654_60min_AUR2_25cm_59ms_1uL_S13.raw	Raw

Items per page:

1 - 5 of 45

Similar Datasets

Project description:Hibernation is energy saving adaptation involving suppression of activity to survive in highly seasonal environments. Immobility and disuse generate muscle loss in most mammalian species. In contrast to other mammals, bears and ground squirrels demonstrate limited muscle atrophy over the physical inactivity of winter hibernation. This suggests that hibernating mammals have adaptive mechanisms to prevent disuse muscle atrophy. To identify common transcriptional program underlying molecular mechanisms preventing muscle loss, we conducted a large-scale gene expression screening in hind limb muscles comparing hibernating and summer active black bears and arctic ground squirrels by the use of custom 9,600 probe cDNA microarrays. The molecular pathway analysis showed an elevated proportion of overexpressed genes involved in all stages of protein biosynthesis and ribosome biogenesis in muscle of both species during hibernation that implies induction of translation at different hibernation states. The induction of protein biosynthesis likely contributes to attenuation of disuse muscle atrophy through prolonged periods of immobility and starvation. This adaptive mechanism allows hibernating mammals to maintain full musculoskeletal function and preserve mobility during and immediately after hibernation, thus promoting survival. The lack of directional changes in genes of protein catabolic pathways does not support the importance of metabolic suppression for preserving muscle mass during winter. Coordinated reduction of multiply genes involved in oxidation reduction and glucose metabolism detected in both species is consistent with metabolic suppression and lower energy demand in skeletal muscle during inactivity of hibernation. Black bears sampled during winter hibernation were compared with the animals sampled during summer. Muscle tissue were hybridized on a custom 12,800 cDNA probe nylon membrane microarray platform . Six hibernating and six summer active bears were studied in the experiment.

Project description:Hibernation is energy saving adaptation involving suppression of activity to survive in highly seasonal environments. Immobility and disuse generate muscle loss in most mammalian species. In contrast to other mammals, bears and ground squirrels demonstrate limited muscle atrophy over the physical inactivity of winter hibernation. This suggests that hibernating mammals have adaptive mechanisms to prevent disuse muscle atrophy. To identify common transcriptional program underlying molecular mechanisms preventing muscle loss, we conducted a large-scale gene expression screening in hind limb muscles comparing hibernating and summer active black bears and arctic ground squirrels by the use of custom 9,600 probe cDNA microarrays. The molecular pathway analysis showed an elevated proportion of overexpressed genes involved in all stages of protein biosynthesis and ribosome biogenesis in muscle of both species during hibernation that implies induction of translation at different hibernation states. The induction of protein biosynthesis likely contributes to attenuation of disuse muscle atrophy through prolonged periods of immobility and starvation. This adaptive mechanism allows hibernating mammals to maintain full musculoskeletal function and preserve mobility during and immediately after hibernation, thus promoting survival. The lack of directional changes in genes of protein catabolic pathways does not support the importance of metabolic suppression for preserving muscle mass during winter. Coordinated reduction of multiply genes involved in oxidation reduction and glucose metabolism detected in both species is consistent with metabolic suppression and lower energy demand in skeletal muscle during inactivity of hibernation. Arctic ground squirrels sampled during winter hibernation were compared with the animals sampled during summer. Muscle was hybridized on a custom 9,600 probes nylon membrane microarray platform. Ten in late torpor, four in early arousal, then in late arousal were studied in experiments.

Dataset Information

Myosin PTM peptide mapping from hibernating and active bears and squirrels

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets