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Complexome profiling of BRAWNIN KO mouse heart mitochondria

ABSTRACT: BRAWNIN (BR) is a mitochondrial microprotein important for assembly of complex III. Here we used complexome to study the composition of supercomplexes in BR KO mouse heart mitochondria. BR deficiency induces the formation of a higher order electron transport chain complex which we renamed as extra-large supercomplex (SC-XL). The stoichiometry of SC-XL is I2+III2. SC-XL formation in the BR KO system alleviates the CIII defects and promotes compensatory OXPHOS increase. It is of higher electron transport efficiency and of lower ROS generation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart

SUBMITTER: Ilka Wittig

LAB HEAD: Lena Ho

PROVIDER: PXD044957 | Pride | 2025-01-08

REPOSITORIES: pride

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			Action	DRS
	Complexome_dataset.xlsx	Xlsx
	Gel.jpg	Other
	P21_015_Singapur_KO_01.raw	Raw
	P21_015_Singapur_KO_02.raw	Raw
	P21_015_Singapur_KO_03.raw	Raw

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