Dataset Information

Activity-Guided Proteomic Profiling of Proteasomes

ABSTRACT: A composition of proteasome complexes in WT yeast, and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1) based on whole-cell proteomic analysis and to activity-guided proteasome profiling of indicates that the amounts of proteasomal proteins and proteasome interacting proteins in the assembled active proteasomes differ significantly from their total amounts in the cell as a whole. This data set is of the activity-guided profiling of the proteasome LFQ analysis.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Oded Kleifeld

LAB HEAD: Oded Kleifeld

PROVIDER: PXD045051 | Pride | 2024-02-15

REPOSITORIES: Pride

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Activity-Guided Proteomic Profiling of Proteasomes Uncovers a Variety of Active (and Inactive) Proteasome Species.

Sahoo Manisha Priyadarsini MP Lavy Tali T Cohen Noam N Sahu Indrajit I Kleifeld Oded O

Molecular & cellular proteomics : MCP 20240129 3

Proteasomes are multisubunit, multicatalytic protein complexes present in eukaryotic cells that degrade misfolded, damaged, or unstructured proteins. In this study, we used an activity-guided proteomic methodology based on a fluorogenic peptide substrate to characterize the composition of proteasome complexes in WT yeast and the changes these complexes undergo upon the deletion of Pre9 (Δα3) or of Sem1 (ΔSem1). A comparison of whole-cell proteomic analysis to activity-guided proteasome profiling ...[more]

PMID: 38296025

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			Action	DRS
	All_experimentalDesignTemplate.txt	Txt
	All_mqpar.xml	Xml
	All_txt.zip	Other
	List_of_Files.txt	Txt
	New_MF_SemiMBR_yeast.zip	Other