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Immunoprecipitation of cellulose synthase complex

ABSTRACT: In order to deepen our understanding of cellulose biosynthesis, this study utilised immunoprecipitation techniques with CESA6 as the bait protein to explore the CSC and its interactors.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Ghazanfar Khan

LAB HEAD: Ghazanfar Khan

PROVIDER: PXD045340 | Pride | 2024-05-23

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	191114_LTI6b1.msf	Msf
	191114_LTI6b1.raw	Raw
	191114_LTi6b2.raw	Raw
	191114_LTi6b3.raw	Raw
	191114_YFP-CESA6-1.raw	Raw

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Publications

Tip Growth Defective1 interacts with the cellulose synthase complex to regulate cellulose synthesis in Arabidopsis thaliana.

Lampugnani Edwin R ER Persson Staffan S Khan Ghazanfar Abbas GA

PloS one 20240215 2

Plant cells possess robust and flexible cell walls composed primarily of cellulose, a polysaccharide that provides structural support and enables cell expansion. Cellulose is synthesised by the Cellulose Synthase A (CESA) catalytic subunits, which form cellulose synthase complexes (CSCs). While significant progress has been made in unravelling CSC function, the trafficking of CSCs and the involvement of post-translational modifications in cellulose synthesis remain poorly understood. In order to ...[more]

PMID: 38358988

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The protein interactomes of AtNHR2A and AtNHR2B unraveled common and specialized functions in plant immunity integrating distinct biological processes

Project description:AtNHR2A (Arabidopsis thaliana nonhost resistance 2A) and AtNHR2B (Arabidopsis thaliana nonhost resistance 2B) are two proteins required in nonhost resistance, a broad spectrum mechanism of plant immunity that protects plants against pathogens. AtNHR2A and AtNHR2B are localized to the cytoplasm, chloroplasts and other subcellular compartments of unknown identity. The multiple localizations of AtNHR2A and AtNHR2B, suggest that they are highly dynamic and versatile proteins likely participating in multiple biological processes. In spite of their importance, their specific functions have not been elucidated. Thus, to aid in the functional characterization of these proteins and identify the biological processes in which these proteins participate, we used immunoprecipitation coupled with mass spectrometry (IP-MS), to identify the proteins interacting with AtNHR2A and AtNHR2B and generate their interactome network. Further analysis of the AtNHR2A- and AtNHR2B-interacting proteins using published empirical information revealed that AtNHR2A and AtNHR2B function is not limited to plant immunity, highlighting that these proteins are hubs integrating defense outputs with other biological processes.

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Fungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase

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Stearic acid blunts growth-factor signaling via oleoylation of GNAI proteins

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