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Proteomic study of salivary gland starvation stress in Hirudo nipponia based on non-labeling quantitative method

ABSTRACT: Hirudo nipponia is an important traditional Chinese medicine resource in China, and the secretions of its salivary glands contain a variety of protein bioactive substances, but due to the low concentration and abundance, they have not been more characterized, and the exploration of its salivary glands is of great significance for the study of the medicinal value and secretion mechanism of leeches. Methods: The expression of differential proteins in salivary gland tissues under starvation (D30) and feeding (D0) states was compared by label-free protein quantification and bioinformatics analysis.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Hirudo Nipponia

TISSUE(S): Salivary Gland

SUBMITTER: Meixiang Cai

LAB HEAD: Meixiang Cai

PROVIDER: PXD045479 | Pride | 2024-07-03

REPOSITORIES: Pride

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Publications

Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia).

Cai Meixiang M Shen Hongying H Xing Yueting Y Wang Weimin W Guan Feng F Luo Yuanyuan Y

PloS one 20240626 6

Hirudo nipponia is an important medicinal animal in China. Its salivary gland secretions contain a variety of protein bioactive substances. Investigations of its salivary glands are of great significance in the study of the medicinal value and mechanism of leech secretions. Illumina RNA-Seq technology was used to perform transcriptome sequencing of salivary gland tissue of H. nipponia under starvation (D30) and fed (D0) states. A total of 2,650 differentially expressed genes (DEGs) were screened ...[more]

PMID: 38923974

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