Proteomics

Dataset Information

0

Whole-cell differential proteomics analysis of MG1655 Escherichia coli (Wild-type and mutants lacking the small regulatory RNA SdsR, OxyS or ZbiJ, or the RNA Chaperone Hfq) experiencing long-term nitrogen starvation in Gutnick minimal media.

ABSTRACT: Proteomics was carried out to compare the proteomes of wild-type MG1655 E coli with mutants lacking either prominent sRNA, SdsR, OxyS or ZbiJ, or lacking the ubiquitous RNA chaperone proteins hfq, in bacteria that had been exposure to long-term (24hr) nitrogen starvation in Gutnick minimal media. The aim of this was to understand what the regulatory contribution of these three sRNA was to bacteria experiencing long-term nitrogen starvation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Escherichia Coli

SUBMITTER: Josh McQuail  

LAB HEAD: Sivaramesh Wigneshweraraj

PROVIDER: PXD045656 | Pride | 2024-05-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
2023-03-13-decoys-contam-UP000000625_Ecoli_strainK12_ID83333_4402entries_27102022_dl11012023.fasta.fas Fasta
FragPipe_workflow.workflow Other
P2514_fractionated_ion.tsv Tabular
P2514_fractionated_peptide.tsv Tabular
P2514_fractionated_protein.tsv Tabular
Items per page:
1 - 5 of 14
altmetric image

Publications

Global Hfq-mediated RNA interactome of nitrogen starved Escherichia coli uncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery.

McQuail Josh J   Matera Gianluca G   Gräfenhan Tom T   Bischler Thorsten T   Haberkant Per P   Stein Frank F   Vogel Jörg J   Wigneshweraraj Sivaramesh S  

Nucleic acids research 20240301 5

The RNA binding protein Hfq has a central role in the post-transcription control of gene expression in many bacteria. Numerous studies have mapped the transcriptome-wide Hfq-mediated RNA-RNA interactions in growing bacteria or bacteria that have entered short-term growth-arrest. To what extent post-transcriptional regulation underpins gene expression in growth-arrested bacteria remains unknown. Here, we used nitrogen (N) starvation as a model to study the Hfq-mediated RNA interactome as Escheric  ...[more]

Similar Datasets

Proteomics Proteomics of HS-5 and NKTert stroma cells
2022-02-17 | PXD027945 | Pride
Proteomics miR-146 modulation in ALS astrocytes
2021-04-06 | PXD024294 | Pride
Proteomics Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex
2022-12-01 | PXD035413 | Pride
Proteomics Targeting IRE1α reprograms the tumor microenvironment and enhances anti-tumor immunity in prostate cancer
2024-08-28 | PXD044287 | Pride
Proteomics Proteome analysis of adipose-derived stem cells cultured under clinically relevant conditions in a wound healing perspective
2016-04-18 | PXD003550 | Pride
Proteomics Proteomic profile of human CHME3 microglia in response to the miR-124-3p modulation in Swedish neuronal cells
2022-06-02 | PXD030315 | Pride
Proteomics A new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
2024-03-05 | PXD048553 | Pride
Proteomics Subcellular proteomics of murine ESC-derived neurons
2022-09-15 | PXD022378 | Pride
Proteomics A combinatorial approach to uncover a novel Integrator subunit
2023-07-20 | PXD039956 | Pride
Proteomics Mapping the protein interaction network of adaptor protein complex in Arabidopsis identifies P34 as a common stability regulator
2023-12-07 | PXD035454 | Pride