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Identification of sialyl Lewisa/x-modified surface proteins in Multiple Myeloma cells by LC-MS

ABSTRACT: The project aims at identifying surface proteins that are modified by the tetrasaccharide sialyl Lewisa/x and therefore serve as selectin ligands in Multiple Myeloma. To that end, we have performed an immunoprecipitation using the Heca452 antibody (which recognizes sialyl Lewisa/x-related structures) and a matched isotype control on membrane-enriched fractions that have been generated from the sialyl Lewisa/x-enriched and parental Multiple Myeloma cell line MM1S and RPMI8226. Using this approach, we have been able to identify candidate proteins that may function as selectin ligands in myeloma.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell, Plasma Cell, Cell Culture

DISEASE(S): Multiple Myeloma

SUBMITTER: Alessandro Natoni

LAB HEAD: Alessandro Natoni

PROVIDER: PXD046178 | Pride | 2024-01-23

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MM1S_Heca452_enriched_Heca452.msf	Msf
	MM1S_Heca452_enriched_Heca452.raw	Raw
	MM1S_Heca452_enriched_Isotype.msf	Msf
	MM1S_Heca452_enriched_Isotype.raw	Raw
	MM1S_Par_Heca452.msf	Msf

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Project description:Human colon cancer cells (HCT-15) treated with 5-aza-2'-deoxycytidine greatly enhanced sialyl Lewis X epitope, which correlated with increased binding of these cells to immobilized E-selectin in a parallel plate dynamic flow binding assay. Human colon cancer cells (HCT-15) treated with 5-aza-2'-deoxycytidine greatly enhanced sialyl Lewis X epitope, which correlated with increased binding of these cells to immobilized E-selectin in a parallel plate dynamic flow binding assay. The sialyl Lewis x epitope resides at a 250 Kd protein band as assessed following immunoprecipitation. We would like to know changes in the expression of glycogenes following 5-aza-2'-deoxycytidine treatment.

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To determine the changes in the expression of human colon cancer cells (HCT-15) untreated and treated with 5-aza-2''-deoxycytidine

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Transcriptomic profile of Sialyl-Lewis X (CD15s)+ basophils and Sialyl Lewis X- basophils

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