Proteomics

Dataset Information

0

An oscillatory transcription factor complex regulates adaptive changes in macropinocytosis during the growth-to-development transition


ABSTRACT: Macropinocytosis is an evolutionarily conserved endocytic pathway that mediates non-selective bulk uptake of extracellular fluid. It is the major route by which axenic Dictyostelium cells obtain nutrients and has emerged as a nutrient-scavenging pathway for mammalian cells. How cells adjust macropinocytic activity in various physiological or developmental contexts is an important question that remains largely unanswered. We discovered that, in Dictyostelium cells, the transcription factors Hbx5 and MybG form a functional complex in the nucleus to maintain macropinocytic activity during the growth stage. In contrast, during starvation-induced multicellular development, the transcription factor complex undergoes nucleocytoplasmic shuttling in response to oscillatory cyclic adenosine 3',5'-monophosphate (cAMP) signals, which leads to increased cytoplasmic retention of the complex and progressive downregulation of macropinocytosis. Therefore, by coupling macropinocytosis-related gene expression to the cAMP oscillation system that facilitates long-range cell-cell communication, the dynamic translocation of the Hbx5-MybG complex orchestrates a population-level adjustment of macropinocytic activity to adapt to changing environmental conditions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Dictyostelium Discoideum (slime Mold)

TISSUE(S): Cell Culture

SUBMITTER: Yazhou Hao  

LAB HEAD: Huaqing Cai

PROVIDER: PXD046585 | Pride | 2024-01-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20191227GFP1.msf Msf
20191227GFP1.raw Raw
20191227GFP2.raw Raw
20191227GFP3.raw Raw
20191227GFP4.raw Raw
Items per page:
1 - 5 of 13

Similar Datasets

2015-07-02 | E-GEOD-63151 | biostudies-arrayexpress
2018-07-03 | PXD001853 | Pride
2024-08-30 | PXD037123 | Pride
2022-03-14 | PXD032072 | Pride
2014-01-03 | GSE47222 | GEO
2024-10-29 | GSE272520 | GEO
2014-01-03 | E-GEOD-47222 | biostudies-arrayexpress
2022-07-29 | PXD032397 | Pride
2021-05-04 | GSE173784 | GEO
2014-04-15 | GSE54866 | GEO