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TOPOVIBL∆C25 mouse protein purification from insect cells

ABSTRACT: The project aims at studying in vitro the TOPOVIBL∆C25 protein. This protein is essential to the formation of DNA double strand breaks, which initiates homologous recombination in meiosis in the mice. We set up a purification procedure of the recombinant TOPOVIBL∆C25 protein expressed in insect cells, which was validated using MS/MS approaches.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Testis, Sperm

SUBMITTER: Functional Proteomics Platform FPP

LAB HEAD: Thomas Robert

PROVIDER: PXD046785 | Pride | 2024-07-09

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	OxidationMSites.txt	Txt
	TOP6MS_210326_HFX_Sample1_100.raw	Raw
	TOP6deltaC25_210326.fasta	Fasta
	allSpectra.HCD.FTMS.iso_0.apl	Other
	allSpectra.HCD.FTMS.iso_1.apl	Other

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1 - 5 of 23

Publications

The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization.

Diagouraga Boubou B Tambones Izabella I Carivenc Coralie C Bechara Chérine C Nadal Marc M de Massy Bernard B le Maire Albane A Robert Thomas T

Nucleic acids research 20240801 15

The TOPOVIL complex catalyzes the formation of DNA double strand breaks (DSB) that initiate meiotic homologous recombination, an essential step for chromosome segregation and genetic diversity during gamete production. TOPOVIL is composed of two subunits (SPO11 and TOPOVIBL) and is evolutionarily related to the archaeal TopoVI topoisomerase complex. SPO11 is the TopoVIA subunit orthologue and carries the DSB formation catalytic activity. TOPOVIBL shares homology with the TopoVIB ATPase subunit. ...[more]

PMID: 38966985

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