Proteomics

Dataset Information

0

Landscape of protein-protein-interactions during hepatitis C virus assembly and release


ABSTRACT: Assembly of infectious hepatitis C virus (HCV) particles requires multiple cellular proteins including for instance apolipoprotein E (ApoE). To describe these protein-protein-interactions, we performed an affinity purification mass spectrometry screen (AP-MS) of HCV infected cells. We used functional viral constructs with epitope-tagged envelope protein 2 (E2), protein (p)7 or nonstructural protein 4B (NS4B), as well as cells expressing a tagged variant of ApoE. We also evaluated assembly stage-dependent remodeling of protein complexes by using viral mutants carrying point mutations abrogating particle production at distinct steps of the HCV particle production cascade. Detected were five ApoE binding proteins, 12 p7 binders, seven primary E2-interactors and 24 proteins interacting with NS4B. Cell-derived PREB, STT3B, SPCS2 as well as viral NS2 interacted with both p7 and E2. Only GTF3C3 interacted with E2 and NS4B, highlighting that HCV assembly and replication complexes exhibit largely distinct interactomes. An HCV core protein mutation, preventing core protein decoration of lipid droplets, profoundly altered the E2 interactome. In cells replicating this mutant, E2 interactions with HSPA5, STT3A/B, RAD23A/B and ZNF860 were significantly enhanced, suggesting that E2 protein interactions partly depend on core protein functions. Bioinformatic and functional studies including STRING network analyses, RNA interference, and ectopic expression support a role of Rad23A and Rad23B in facilitating HCV infectious virus production. Both Rad23A and Rad23B are involved in the endoplasmic reticulum (ER)-associated protein degradation (ERAD). Collectively, our results provide a map of host proteins interacting with HCV assembly proteins and they give evidence for the involvement of ER protein folding machineries and the ERAD pathway in the late stages of the HCV replication cycle.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mario Oroshi  

LAB HEAD: Felix Meissner

PROVIDER: PXD046941 | Pride | 2023-12-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MutantIPs_RAW.zip Other
Mutant_search.zip Other
VirusIPs_RAW.zip Other
Virus_search.zip Other
Items per page:
1 - 4 of 4

Similar Datasets

2014-02-09 | E-GEOD-54722 | biostudies-arrayexpress
2024-05-21 | PXD052401 | Pride
2016-01-21 | PXD001992 | Pride
2012-10-22 | E-GEOD-40224 | biostudies-arrayexpress
2011-01-25 | GSE26838 | GEO
2018-04-09 | PXD009007 | Pride
2011-01-25 | E-GEOD-26838 | biostudies-arrayexpress
2010-06-25 | E-GEOD-18084 | biostudies-arrayexpress
2012-10-22 | E-GEOD-40184 | biostudies-arrayexpress
2014-03-07 | E-GEOD-55677 | biostudies-arrayexpress