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Protein turnover dynamics of IAV-infected HeLa cells

ABSTRACT: The abundance of a protein is defined by its continuous synthesis and degradation, a process known as protein turnover. Here, we systematically profiled the turnover of proteins in influenza A virus (IAV) infected cells using a pulse-chase SILAC-based approach combined with downstream statistical modeling. We identified 1414 virus-affected proteins with turnover changes (tVAPs) out of 7739 detected proteins. In particular, the affected proteins were involved in RNA transcription, splicing and nuclear transport, protein translation and stability, and energy metabolism. Many tVAPs are known IAV-interacting proteins that regulate virus propagation, such as KPNA6, PPP6C and POLR2A. Notably, our analysis identified novel IAV host and restriction factors, such as the splicing factor GPKOW, that exhibit significant turnover rate changes, while their total abundance is not affected. Overall, we show that protein turnover is a critical factor both for virus replication and antiviral defense.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

DISEASE(S): Influenza

SUBMITTER: Yiqi Huang

LAB HEAD: Andreas Pichlmair

PROVIDER: PXD047063 | Pride | 2025-04-09

REPOSITORIES: Pride

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			Action	DRS
	20140728_QEp5_PhHu_SA_Ph_PU_22_Mock1_SAX_1.raw	Raw
	20140728_QEp5_PhHu_SA_Ph_PU_22_Mock1_SAX_2.raw	Raw
	20140728_QEp5_PhHu_SA_Ph_PU_22_Mock1_SAX_3.raw	Raw
	20140728_QEp5_PhHu_SA_Ph_PU_22_Mock1_SAX_4.raw	Raw
	20140728_QEp5_PhHu_SA_Ph_PU_22_Mock1_SAX_5.raw	Raw

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