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Consecutive digestion of archaeological skeletal proteomes with multiple proteases

ABSTRACT: Ancient skeletal proteomes are increasingly utilised for phylogenetic and evolutionary analysis. These proteomes are, however, often small and with low sequence coverage. We expand on previous observations which have shown that parallel digestion of Pleistocene skeletal proteomes increases proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive digestion of a skeletal proteome using two proteases, particularly Glu-C or chymotrypsin followed by trypsin digestion, enables the recovery of alternative proteome components not reachable through trypsin digestion alone. The sequential utilisation of several proteases is a promising avenue for the study of highly degraded ancient proteomes for phylogenetic purposes.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Equus Caballus (horse) Bos Taurus (bovine)

TISSUE(S): Bone

SUBMITTER: Zandra Fagernäs

LAB HEAD: Frido Welker

PROVIDER: PXD047069 | Pride | 2024-03-06

REPOSITORIES: Pride

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			Action	DRS
	20221115_EXPL5_nLC3_GT_collab_77min_DDA_Blank_P0208.raw	Raw
	20221115_EXPL5_nLC3_GT_collab_77min_DDA_Bone_P0196.raw	Raw
	20221115_EXPL5_nLC3_GT_collab_77min_DDA_Bone_P0197.raw	Raw
	20221115_EXPL5_nLC3_GT_collab_77min_DDA_Bone_P0198.raw	Raw
	20221115_EXPL5_nLC3_GT_collab_77min_DDA_Bone_P0199.raw	Raw

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