Comparative Proteomic AnalyComparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patientssis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patients

ABSTRACT: The pathogenesis of myopia involves different molecular mechanisms and pathways, which can be reflected in the aqueous humor. In our study, aqueous humor was collected for liquid chromatograph mass spectrometer (LC/MS) analysis from 30 individual eyes that underwent phacoemulsification and intraocular lens (IOL) implantation. A total of 190 DEPs (differentially expressed proteins) were identified, which revealed their involvement in pathways such as carbon metabolism, lipid, and cholesterol metabolism, ECM (extracellular matrix) component regulation, and scleral remodeling in the context of high myopia. Further analysis revealed 15 interacting hub proteins, encompassing functions related to lipoprotein assembly, regulation, complement cascades, and fibrin formation. Subsequent validation demonstrated elevated levels of APOE (apolipoprotein E), C3 (complement 3), and AHSG (alpha-2-HS-glycoprotein) in the high myopia group (31 eyes of cataracts and 45 eyes of high myopia with cataracts), while AHSG had a significant positive correlation with axial length in patients with high myopia and good efficacy in distinguishing between myopic and non-myopic groups. These results suggested that the interaction of lipoproteins, complement cascades, and the fibrinolytic system played a pivotal role in the pathological process of myopia, while the metabolism of essential materials and the remodeling of ECM and tissue structure were affected in the eyes of individuals with high myopia.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Anterior Chamber Of The Eye

DISEASE(S): Myopia

SUBMITTER: Jingxiao Du  

LAB HEAD: Zhihua Zhang

PROVIDER: PXD047584 | Pride | 2024-05-23

REPOSITORIES: Pride