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Human corneal stroma proteomic profile of myopic eyes

ABSTRACT: This is a cross-sectional case-control study. Study subjects of generalized myopic patients in preparation for small incision lenticule extraction (SMILE) surgery were recruited and divided into low and high myopia subgroups.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cornea, Keratocyte

DISEASE(S): Myopia

SUBMITTER: Yan Wang

LAB HEAD: Yan Wang

PROVIDER: PXD025145 | Pride | 2022-02-17

REPOSITORIES: Pride

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			Action	DRS
	P20190701059-F1.raw	Raw
	P20190701059-F10.raw	Raw
	P20190701059-F11.raw	Raw
	P20190701059-F12.raw	Raw
	P20190701059-F13.raw	Raw

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Publications

Novel Corneal Protein Biomarker Candidates Reveal Iron Metabolic Disturbance in High Myopia Eyes.

Chen Jingyi J Wu Wenjing W Wang Zhiqian Z Zhai Chuannan C Deng Baocheng B Alzogool Mohammad M Wang Yan Y

Frontiers in cell and developmental biology 20211001

Myopia is a major public health concern with increasing global prevalence and is the leading cause of vision loss and complications. The potential role of the cornea, a substantial component of refractive power and the protective fortress of the eye, has been underestimated in the development of myopia. Our study acquired corneal stroma tissues from myopic patients undergoing femtosecond laser-assisted small incision lenticule extraction (SMILE) surgery and investigated the differential expressi ...[more]

PMID: 34660571

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microRNA profiling in the aqueous humor of highly myopic eyes by next generation sequencing

Project description:Purpose: To characterize microRNAs (miRNAs) and their possible roles in high myopia by using next generation sequencing Methods: Aqueous humor samples were obtained from 15 highly myopic eyes and 15 cataract eyes at the onset of surgery. miRNA next generation sequencing and bioinformatics analyses were performed using RNA extracted from aqueous humor samples. Results: A total of 341 miRNAs were detected in the aqueous humor samples of highly myopic eyes; 201 miRNAs were detected in the aqueous humor samples of cataractous control eyes. A total of 249 mature miRNAs and 17 novel miRNAs were differentially expressed during myopia. Possible pathways regulated by these aberrantly expressed miRNAs included the TNF, MAPK, PI3K-Akt, and HIF-1 signaling pathways. Conclusions: The current study provided an overall view of miRNA profiling in the aqueous humor of highly myopic eyes. These profiles may be associated with myopia pathogenesis, and are potential biomarkers.

2021-12-15 | GSE142359 | GEO

Comparative Proteomic AnalyComparative Proteomic Analysis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patientssis of Aqueous Humor Reveals Biochemical Disparities in Eyes of High Myopic Patients

Project description:The pathogenesis of myopia involves different molecular mechanisms and pathways, which can be reflected in the aqueous humor. In our study, aqueous humor was collected for liquid chromatograph mass spectrometer (LC/MS) analysis from 30 individual eyes that underwent phacoemulsification and intraocular lens (IOL) implantation. A total of 190 DEPs (differentially expressed proteins) were identified, which revealed their involvement in pathways such as carbon metabolism, lipid, and cholesterol metabolism, ECM (extracellular matrix) component regulation, and scleral remodeling in the context of high myopia. Further analysis revealed 15 interacting hub proteins, encompassing functions related to lipoprotein assembly, regulation, complement cascades, and fibrin formation. Subsequent validation demonstrated elevated levels of APOE (apolipoprotein E), C3 (complement 3), and AHSG (alpha-2-HS-glycoprotein) in the high myopia group (31 eyes of cataracts and 45 eyes of high myopia with cataracts), while AHSG had a significant positive correlation with axial length in patients with high myopia and good efficacy in distinguishing between myopic and non-myopic groups. These results suggested that the interaction of lipoproteins, complement cascades, and the fibrinolytic system played a pivotal role in the pathological process of myopia, while the metabolism of essential materials and the remodeling of ECM and tissue structure were affected in the eyes of individuals with high myopia.

2024-05-23 | PXD047584 | Pride

Serological autoantibody profiling of emmetropic controls and highly myopic patiens

Project description:High myopia is a leading cause of blindness worldwide, among which pathologic myopia, characterized by typical myopic macular degeneration, is the most detrimental. However, its pathogenesis remains largely unknown. By using a protein microarray assaying more than 20,000 human proteins, we determined in this study the autoantibody profiles of highly myopic patiens and emmetropic controls.

2024-05-08 | GSE223434 | GEO

High throughput gene expression analysis identifies reliable expression markers of human corneal endothelial cells.

Project description:Considerable interest has been generated for the development through cell-tissue engineering of suitable corneal endothelial graft alternatives, which can potentially alleviate the shortage of corneal transplant material. The advent of less invasive suture-less key-hole surgery options such as DescemetM-bM-^@M-^Ys Stripping Endothelial Keratoplasty (DSEK) and DescemetM-bM-^@M-^Ys Membrane Endothelial Keratoplasty (DMEK), which involve transplantation of solely the endothelial layer instead of full thickness cornea, provide further impetus for the development of alternative endothelial grafts for clinical applications. A major challenge for this endeavor is the lack of specific markers for this cell type. To identify genes that reliably mark corneal endothelial cells (CECs) in vivo and in vitro, we performed RNA-sequencing on freshly isolated human CECs (from both young and old donors), CEC cultures, and corneal stroma. Gene expression of these corneal cell types were also compared to that of other human tissue types. Based on high throughput comparative gene expression analysis, we identified a panel of markers that are: i) highly expressed in CECs from both young donors and old donors; ii) expressed in CECs in vivo and in vitro; and iii) not expressed in corneal stroma keratocytes and the activated corneal stroma fibroblasts. These were SLC4A11, COL8A2 and CYYR1. The use of this panel of genes in combination reliably ascertains the identity of the CEC cell type. A total of 20 donor corneas consisting of 10 single donor corneas and 5 paired donor corneas were used in this study. Donor age ranged from 19 - 76. This RNA-seq study included 15 pooled corneas (5 each) used form CEC old, CEC young and stroma samples.

2013-05-23 | E-GEOD-44064 | biostudies-arrayexpress

Evaluation of MicroRNA Expression Profiles for Form-Deprivation Myopia in Mouse

Project description:The study aim was to determine microRNA (miRNA) expression profiles of ocular tissues in form-deprivation induced myopia (FDM) mice. Form-deprivation myopia was induced in C57BL/6Jmice over the right eye; the contralateral left eyes were used as controls. Whole genome microRNA expression profiles in myopic whole eye, retina, and sclera were determined using the Agilent mouse miRNA microarray. The normalized microarray data were performed ANOVA test to identify differences in miRNA expression between myopic and control eyes. The differential expression for selected miRNAs was validated by quantitative real time PCR (qRT-PCR).

2014-12-31 | GSE58124 | GEO

Large-scale microRNA expression profiling identifies retinal miRNA-mRNA signaling pathways underlying form-deprivation myopia in mice

Project description:Development of myopia is associated with large-scale changes in ocular tissue gene expression. Although differential expression of coding genes underlying development of myopia has been a subject of intense investigation, the role of non-coding genes such as microRNAs in the development of myopia is largely unknown. In this study, we explored myopia-associated miRNA expression profiles in the retina and sclera of C57Bl/6J mice with experimentally induced myopia using microarray technology. We found a total of 53 differentially expressed miRNAs in the retina and no differences in miRNA expression in the sclera of C57BL/6J mice after 10 days of visual form deprivation, which induced -6.93 ± 2.44 D (p < 0.000001, n = 12) of myopia. We also identified their putative mRNA targets among mRNAs found to be differentially expressed in myopic retina and potential signaling pathways involved in the development of form-deprivation myopia using miRNA-mRNA interaction network analysis. Analysis of myopia-associated signaling pathways revealed that myopic response to visual form deprivation in the retina is regulated by a small number of highly integrated signaling pathways. Our findings highlight substantial involvement of miRNAs in the regulation of refractive eye development, and in the development of myopia through the retinal gene regulation.

2017-02-09 | GSE84220 | GEO

Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia

Project description:Myopia has become the major cause of visual impairment worldwide. Although the pathogenesis of myopia remains controversial, proteomics studies suggest that dysregulation of retinal metabolism is potentially involved in the pathology of myopia. Lysine lactylation of proteins plays a key role in regulating cellular metabolism, but little is known about its role in the form-deprived myopic retina. In this study, we performed lactylation proteomic analysis of the retinas of form-deprived myopic guinea pigs and found downregulated levels of lactylation of metabolism-critical enzymes in the retina. As the first report on retinal lactylation in myopic eyes, this study provides a reliable basis for further studies on retinal lactylation in myopic eyes.

2024-10-17 | PXD046495 | Pride

Widespread Involvement of Acetylation in the Retinal Metabolism of Form-Deprivation Myopia in Guinea Pigs

Project description:Myopia has become the major cause of visual impairment worldwide. Although the pathogenesis of myopia remains controversial, proteomics studies suggest that dysregulation of retinal metabolism is potentially involved in the pathology of myopia. Lysine acetylation of proteins plays a key role in regulating cellular metabolism, but little is known about its role in the form-deprived myopic retina. In this study, we performed acetylation proteomic analysis of the retinas of form-deprived myopic guinea pigs and found downregulated levels of acetylation of metabolism-critical enzymes in the retina. As the first report on retinal acetylation in myopic eyes, this study provides a reliable basis for further studies on retinal acetylation in myopic eyes

2023-07-12 | PXD043196 | Pride

Proteomic analysis of pro-stromal corneal tissue of highly myopic males and females

Project description:In this study, we aimed to explore the sex differences in myopic cornea using corneal stromal removed by small incision lenticule extraction (SMILE) surgery. The corneal stroma of highly myopic female and highly myopic male were subjected to proteomic assays. The total number of proteins in the cornea and the proteins specifically expressed in the two groups were counted, and the differentially expressed proteins in the two groups were identified by expression fold change >2 and P value <0.05, and volcano plots were constructed, functional enrichment analysis, and molecular interaction were implemented. Finally, 10 samples from each group were analyzed. 27 down-regulated proteins and 27 up-regulated proteins in the FH group, of which 23 proteins were up-regulated by a factor of 2-10 and 4 proteins were up-regulated by a factor of greater than 10. And the increased expression of proteins associated with extracellular matrix and collagen I, III, V, and VIII in female patients, and the TGF-β1/Smad3 pathway was an important pathway obtained from functional analysis. The findings of this study may help to identify sex differences that may exist in myopic corneas，and may help to further explore the pathways of myopia development and thus provide ideas for future treatments.

2024-08-06 | PXD054620 |

Transcription profiling of mouse primary cultures from mouse cornea vs those of the adult and 10-day postnatal mouse cornea to elucidate biological processes underlying the keratocyte, fibroblast, and myofibroblast phenotypes of corneal stromal cells

Project description:To elucidate biological processes underlying the keratocyte, fibroblast, and myofibroblast phenotypes of corneal stromal cells, the gene expression patterns of these primary cultures from mouse cornea were compared with those of the adult and 10-day postnatal mouse cornea.

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