Proteomics

Dataset Information

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Thermothielavioides terrestris secretome after growth on raw and mildly steam-pretreated spruce


ABSTRACT: One important obstacle in using softwood in biorefineries is its recalcitrance to enzymatic saccharification. In the present work, Thermothielavioides terrestris LPH172 was cultivated on three different steam-pretreated spruce materials and on non-treated spruce, harboring different hemicellulose content and structural characteristics. The aim of the work was to map the substrate-induced changes in the secretome of T. terrestris when grown on differently treated spruce materials and evaluate the hydrolytic efficiency of the secretome as supplement of a commercial enzyme mixture. The cultivation of T. terrestris was monitored by enzyme activity measurements of endo-cellulase, endo-xylanase, endo-mannanase, laccase and peroxidase. Proteomic analysis was performed on the secretomes induced by these materials to map the differences in enzyme expression.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Thermothielavioides Terrestris

SUBMITTER: Romanos Siaperas  

LAB HEAD: Lisbeth Olsson

PROVIDER: PXD047620 | Pride | 2024-10-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
4479_Lumos_220902_59to78.mzTab.gz Mztab
Lumos_220902_59.raw Raw
Lumos_220902_60.raw Raw
Lumos_220902_61.raw Raw
Lumos_220902_62.raw Raw
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Publications

Elucidating Thermothielavioides terrestris secretome changes for improved saccharification of mild steam-pretreated spruce.

Caputo Fabio F   Siaperas Romanos R   Dias Camila C   Nikolaivits Efstratios E   Olsson Lisbeth L  

Biotechnology for biofuels and bioproducts 20241005 1


<h4>Background</h4>The efficient use of softwood in biorefineries is hampered by its recalcitrance to enzymatic saccharification. In the present study, the fungus Thermothielavioides terrestris LPH172 was cultivated on three steam-pretreated spruce materials (STEX<sub>180°C/auto</sub>, STEX<sub>210°C/auto</sub>, and STEX<sub>210°C/H2SO4</sub>), characterized by different hemicellulose content and structure, as well as on untreated biomass. The aim of the study was to map substrate-induced change  ...[more]

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