Project description:We extracted mitochondria from Hela cells which were treated with CCCP or DMSO for 2 h. The samples were digested by FAST and peptides were used DIA to analysis.
Project description:In order to determine proteins interacting with ARID3A in the ML-DS cell line CMK, we performed CoIP of endogenous ARID3A followed by LC-MS/MS
Project description:To investigate whether GltB possess more function, Co-immunoprecipitation coupled with Mass Spectrometry (CoIP-MS) was used to isolate the putative GltB binding proteins.We used strains PAO1/p-gtrS-YFP and ΔgltB/p-gtrS-YFP to ensure the membrane protein GtrS of great interest is at high levels. A target GltB-specific antibody anti-GltB was used and ΔgltB/p-gtrS-YFP was set up as a negative control. The whole gel lane was divided into small fractions and in-gel digested for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Control sample was also analyzed in parallel to distinguish the background proteins.
Project description:We extracted DIP from placanta tissue of human which were diagnose preeclampsia or normal. The samples of DIPs were digested by FAST and the peptides were used DIA to analysis.
Kun Meng