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The PRC1 and PRC2 proximal interactome: the link between H4ac an H3K27me3

ABSTRACT: Polycomb Repressive Complexes PRC1 and PRC2 play a crucial role in silencing lineage-specific genes during early embryogenesis. To provide new insights in polycomb biology, we profiled the proximal interactome (proxeome) of the catalytic subunits RNF2 (PRC1) and EZH2 (PRC2) in mouse embryonic stem cells (mESCs). This revealed >100 proteins proximal to PRC12 and PRC21, which mainly comprise transcription factors, transcriptional regulators and RNA binding proteins. To investigate the link between PRC2 and NuA4, and therefore the the link between H4ac an H3K27me3, we perturbed PRC2 both chemically and genetically.The effects on the histone PTMs were measured with LC-MS/MS.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell, Cell Culture

SUBMITTER: Maarten Dhaenens

LAB HEAD: Dr Maarten Dhaenens

PROVIDER: PXD048246 | Pride | 2025-02-25

REPOSITORIES: pride

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			Action	DRS
	211115_DDA_H4ac_1_1.wiff	Wiff
	211115_DDA_H4ac_1_1.wiff.scan	Wiff
	211115_DDA_H4ac_1_2.wiff	Wiff
	211115_DDA_H4ac_1_2.wiff.scan	Wiff
	211115_DDA_H4ac_1_3.wiff	Wiff

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