Dataset Information

High-Throughput Identification of Calcium Regulated Proteins Across Diverse Proteomes

ABSTRACT: Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by lack of high-throughput, unbiased, and quantitative methods to identify proteins-calcium engagement. To address this, we adapted protein thermostability assays in the budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. These data revealed calcium engagement of novel signaling hubs and cellular processes, including metabolic enzymes and the spliceosome. Cross-species comparison of calcium-protein engagement and mutagenesis experiments identified residue-specific cation engagement, even within well-known EF-hand domains. Additionally, we found that the dienoyl-CoA reductase DECR1 binds calcium at physiologically-relevant concentrations with substrate-specific affinity, suggesting direct calcium regulation of mitochondrial fatty acid oxidation. These unbiased, proteomic analyses of calcium effectors establish a key resource to dissect cation engagement and its mechanistic effects across multiple species and diverse biological processes.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hek-293t Cell, Cell Culture

SUBMITTER: Devin Schweppe

LAB HEAD: Devin Schweppe

PROVIDER: PXD048653 | Pride | 2024-11-06

REPOSITORIES: Pride

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			Action	DRS
	220929_HEK_MBP_PISA_4_4_A_Frac_eca4387.mzXML	Mzxml
	220929_HEK_MBP_PISA_4_4_A_Frac_eca4388.mzXML	Mzxml
	220929_HEK_MBP_PISA_4_4_A_Frac_eca4389.mzXML	Mzxml
	220929_HEK_MBP_PISA_4_4_A_Frac_eca4390.mzXML	Mzxml
	220929_HEK_MBP_PISA_4_4_A_Frac_eca4391.mzXML	Mzxml

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Publications

High-throughput identification of calcium-regulated proteins across diverse proteomes.

Locke Timothy M TM Fields Rose R Gizinski Hayden H Otto George M GM MacEwen Melissa J S MJS Rusnac Domnita-Valeria DV He Peixian P Shechner David M DM McGann Chris D CD Berg Matthew D MD Villen Judit J Sancak Yasemin Y Schweppe Devin K DK

Cell reports 20241018 11

Calcium ions play important roles in nearly every biological process, yet whole-proteome analysis of calcium effectors has been hindered by a lack of high-throughput, unbiased, and quantitative methods to identify protein-calcium engagement. To address this, we adapted protein thermostability assays in budding yeast, human cells, and mouse mitochondria. Based on calcium-dependent thermostability, we identified 2,884 putative calcium-regulated proteins across human, mouse, and yeast proteomes. Th ...[more]

PMID: 39425928

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