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Phosphoproteomics of Hela cells stimulated with EGF in the presence of protein phosphatase inhibitors

ABSTRACT: We profiles the effects of six protein phosphatase inhibitors on EGF-dependent phosphoproteome dynamics. The cells were treated with 20 nM EGF for 8 or 20 min with or without 15 min pre-treatment of one of the phosphatase inhibitors (100 nM or 1 M; Raphin1 acetate, sanguinarine chloride, KY-226, SHP099, NSC95397, or BCI). We further focused on sanguinarine chloride, and Hela cells were treated with 20 nM EGF for 3, 8, or 20min with or without 15 min pre-treatment of sanguinarine (5, 50, or 500 nM). We also examined combination of sanguinarine chloride (500 nM) and BIRB796 (500 nM), a p38-kinase inhibitor.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Akihiro Eguchi

LAB HEAD: Jesper. V. Olsen

PROVIDER: PXD049123 | Pride | 2024-04-30

REPOSITORIES: Pride

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			Action	DRS
	20221020_EXPL1_Evo2_AE_SA_60spd_DIA_phospho_HeLa_Cdc_100nM_20_R1_O27.raw	Raw
	20221020_EXPL1_Evo2_AE_SA_60spd_DIA_phospho_HeLa_Cdc_100nM_20_R2_O14.raw	Raw
	20221020_EXPL1_Evo2_AE_SA_60spd_DIA_phospho_HeLa_Cdc_100nM_20_R3_O1.raw	Raw
	20221020_EXPL1_Evo2_AE_SA_60spd_DIA_phospho_HeLa_Cdc_100nM_20_R4_O2.raw	Raw
	20221020_EXPL1_Evo2_AE_SA_60spd_DIA_phospho_HeLa_Cdc_100nM_20_R5_O27.raw	Raw

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