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Identification of phosphorylation sites on NAP1/AZI2

ABSTRACT: We observed smearing for the TBK1-adaptor protein NAP1/AZI2 during mitophagy. To verify whether this smearing was due to phosphorylation by TBK1, we performed an in vitro kinase assay and complemented this with the immunoprecipitation of NAP1 from HAP1, whose genetic background (FIP200 knockout) results in hyperactivation of TBK1.

INSTRUMENT(S): Q Exactive HF-X, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: WeiQiang Chen

LAB HEAD: Sascha Martens

PROVIDER: PXD049184 | Pride | 2024-04-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20211015_E2_RSLC2_Adriaenssens_Martens_MPL_UniWien_NAP_30min_CT_50p.raw	Raw
	20211015_E2_RSLC2_Adriaenssens_Martens_MPL_UniWien_NAP_30min_T_50p.raw	Raw
	20211105_E3_RSLC6_Adriaenssens_Martens_MPL_UniWien_CoIP_CT_50p.raw	Raw
	20211105_E3_RSLC6_Adriaenssens_Martens_MPL_UniWien_CoIP_T_50p.raw	Raw
	20211105_QExHFX2_RSLC10_Adriaenssens_Martens_MPL_UniWien_CoIP_CT_50p.raw	Raw

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