MS-based proteomic characterization of assembled microtubules
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ABSTRACT: Microtubules are dynamic polymers that interconvert between phases of growth and shrinkage, yet they somehow provide lasting structural stability to cells. Growth involves hydrolysis of GTP-tubulin to GDP-tubulin, which releases energy that is stored within the microtubule lattice and destabilizes it; a GTP cap at microtubule ends is thought to prevent GDP subunits from rapidly dissociating and causing catastrophe. We show that GDP-tubulin, usually considered as an inactive tubulin species, can itself assemble into microtubules preferentially at the minus end, and promote persistent growth. We characterized by mass spectrometry-based proteomics the protein content of tubulin purified from bovine brain (Total) and of microtubules grown from stable GMPCPP seeds in the presence of either GDP-tubulin or GTP-tubulin.
INSTRUMENT(S): Q Exactive HF
ORGANISM(S): Bos Taurus (bovine)
TISSUE(S): Brain
SUBMITTER: Yohann Couté
LAB HEAD: Yohann Couté
PROVIDER: PXD049371 | Pride | 2024-04-30
REPOSITORIES: Pride
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