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Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi

ABSTRACT: Various small RNA libraries from purified microtubules or Xiwi immunoprecipitates or total extract from X.tropicalis or X.laevis egg extract. Various small RNA libraries from single X.tropicalis eggs. Analysis of this series of files is described in the manuscript "Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi". Small RNAs were ligated with linkers and converted to cDNA by reverse transcription. cDNA library was amplified by PCR and was sequenced with either the 454 Genome Sequencer FLX platform or the Illumina GA-II platform.

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Nelson Lau

PROVIDER: E-GEOD-15556 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematic and single cell analysis of Xenopus Piwi-interacting RNAs and Xiwi.

Lau Nelson C NC Ohsumi Toshiro T Borowsky Mark M Kingston Robert E RE Blower Michael D MD

The EMBO journal 20090827 19

Piwi proteins and Piwi-interacting RNAs (piRNAs) are essential for germ cell development, but analysis of the molecular mechanisms of these ribonucleoproteins remains challenging in most animal germ cells. To address this challenge, we systematically characterized Xiwi, a Xenopus Piwi homologue, and piRNAs from Xenopus eggs and oocytes. We used the large size of Xenopus eggs to analyze small RNAs at the single cell level, and find abundant piRNAs and large piRNA clusters in the Xenopus tropicali ...[more]

PMID: 19713941

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