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Complete list of canonical post-transcriptional modifications in Bacillus subtilis ribosome.

ABSTRACT: Using bottom-up oligonucleotide LC-MS/MS in combination with stable isotope labeling, chemical derivatization, and bioinformatics variable modification search, a total of 25 modifications were mapped to the sequences of 16S and 23S RNA in B. subtilis. 10 modification sites, previously identified by others using alternative methods were independently confirmed using targeted MS/MS data analysis. Modification types, namely base/ribose methylation, pseudouridines and dihydrouridine, and modification positions are fully consistent with prior experimental evidence and agree well with modifications that were annotated in E. coli and other bacteria species.

INSTRUMENT(S): 6520A Quadrupole Time-of-Flight LC/MS, impact II

ORGANISM(S): Bacillus Subtilis Py79 Bacteria

SUBMITTER: Anna Popova

LAB HEAD: James Williamson

PROVIDER: PXD050367 | Pride | 2024-08-06

REPOSITORIES: Pride

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			Action	DRS
	16S_23S_MSMS_spectra_annotation.xlsx	Xlsx
	16S_RNaseH_data.zip	Other
	16S_RNaseH_search.zip	Other
	16S_RNases_T1_A_data.zip	Other
	16S_RNases_T1_A_search.zip	Other

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