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Streamlined analysis of drug targets by proteome integral solubility alteration indicates organ-specific engagement

ABSTRACT: Proteins are the primary targets of almost all small molecule drugs. However, even the most selectively designed drugs can potentially target several unknown proteins. Identification of potential drug targets can facilitate design of new drugs and repurposing of existing ones. Current state-of-the-art proteomics methodologies enable screening of thousands of proteins against a limited number of drug molecules. Here we report the development of a label-free quantitative proteomics approach that enables proteome-wide screening of small organic molecules in a scalable, reproducible, and rapid manner by streamlining the proteome integral solubility alteration (PISA) assay. We used rat organs ex-vivo to determine organ specific targets of medical drugs and enzyme inhibitors to identify novel drug targets for common drugs such as Ibuprofen. Finally, global drug profiling revealed overarching trends of how small molecules affect the proteome through either direct or indirect protein interactions.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human)

TISSUE(S): Spleen, Brain, Liver, Cell Culture, Kidney, Hela Cell

SUBMITTER: Tanveer Batth

LAB HEAD: Jesper Velgaard

PROVIDER: PXD050784 | Pride | 2024-10-16

REPOSITORIES: Pride

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			Action	DRS
	DIA-NN_analysis.zip	Other
	FASTA_and_spectral_libraries.zip	Other
	PISA_HeLa_HepG2.zip	Other
	Pilot_experiments.zip	Other
	Rat_organ_Cancer_Drugs.zip	Other

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