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Annotation of the genes responsible for helix 69 rRNA modifications in 23S of Bacillus subtilis.

ABSTRACT: A total of 25 modifications were mapped to 16S and 23S RNA in B. subtilis ribosome, however most of the genes responsible for these modification sites remain unknown. In this work, bottom-up oligonucleotide LC-MS/MS was used to detect rRNA modifications in five single gene knock-out strains: yydA, yhcT, yjbO, ylyB, yqxC. By comparing oligonucleotide modification states between the background 168 strain (annotated as WT) and the mutants: ylyB was confirmed to be a pseudouridine synthase at positions 1940, 1944, 1946 of 23S; yydA is a N3-pseudouridine methyltransferase at 1944 of 23S; and yqxC is a dual 2’O-cytidine methyltransferase at 1417 of 16S and 1949 of 23S. Deletion of yhcT or yjbO does not change rRNA modification pattern.

INSTRUMENT(S): impact II

ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. 168

SUBMITTER: Anna Popova

LAB HEAD: James Williamson

PROVIDER: PXD051518 | Pride | 2024-08-06

REPOSITORIES: Pride

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			Action	DRS
	70S_A_db_search.zip	Other
	70S_A_mgf_peak_list.zip	Other
	70S_A_raw_data.zip	Other
	70S_T1_db_search.zip	Other
	70S_T1_mgf_peak_list.zip	Other

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