Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ENL reads histone β-hydroxybutyrylation to modulate gene transcription

ABSTRACT: Identification of hPTM binder through the chemoproteomic approach

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hepatocyte

DISEASE(S): Disease Free

SUBMITTER: Feng Zhai

LAB HEAD: Guijin Zhai

PROVIDER: PXD051689 | Pride | 2024-06-26

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	H3K4-1.raw	Raw
	H3K4-2.raw	Raw
	H3K4-3.raw	Raw
	H3K4me3-1.raw	Raw
	H3K4me3-2.raw	Raw

Items per page:

1 - 5 of 14

Similar Datasets

DPF2 reads histone lactylation to drive transcription and tumorigenesis

Project description:Identification of hPTM binder through the chemoproteomic approach

2024-12-23 | PXD051995 | Pride

ENL reads histone β-hydroxybutyrylation to modulate gene transcription (RNA-Seq)

Project description:Histone modifications are typically recognized by chromatin-binding protein modules (referred to as “readers”) to mediate fundamental processes such as transcription. Lysine β-hydroxybutyrylation (Kbhb) is a new type of histone mark that couples metabolism to gene expression. However, the readers that prefer histone Kbhb remain elusive. This knowledge gap must be filled in order to reveal the molecular mechanism of this epigenetic regulation. Herein, we developed a chemical proteomic approach, relying upon multivalent photoaffinity probes to capture binders of the mark and identified ENL as a novel target of H3K9bhb. Biochemical studies and CUT&Tag analysis further suggested that ENL favorably binds to H3K9bhb, and co-localizes with it on promoter regions to modulate gene expression. Notably, disrupting the interaction between H3K9bhb and ENL via structure-based mutation leads to the suppressed expression of the gene like MYC that drives cell proliferation.

2024-05-06 | GSE266269 | GEO

ENL reads histone β-hydroxybutyrylation to modulate gene transcription (CUT&Tag)

2024-05-06 | GSE266268 | GEO

ENL reads histone β-hydroxybutyrylation to modulate gene transcription (CUT&Tag)

Project description:ENL reads histone β-hydroxybutyrylation to modulate gene transcription (CUT&Tag)

| PRJNA1106475 | ENA

ENL reads histone β-hydroxybutyrylation to modulate gene transcription (RNA-Seq)

Project description:ENL reads histone β-hydroxybutyrylation to modulate gene transcription (RNA-Seq)

| PRJNA1106487 | ENA

Physiological tau interactome in brain and its link to tauopathies_complete submission

Project description:Alzheimer's disease (AD) and most of other tauopathies are incurable neurodegenerative diseases with unpleasant symptoms and consequences. The common hallmark of all these diseases is tau pathology but its connection with disease progress has not been completely understood so far. Therefore, uncovering novel tau interacting partners and pathology affected molecular pathways can reveal the causes of diseases as well as potential targets for development of AD treatment. Despite of large amount of known tau interacting partners, limited number of studies focused on in vivo tau interactions in disease or healthy conditions are available. Here, we applied an in vivo crosslinking approach, capable of capturing weak and transient protein-protein interactions, to a unique transgenic rat model of progressive tau pathology similar to human AD. We have identified 175 potential novel and known tau interacting proteins by MALDI-TOF mass spectrometry. Several of the most promising candidates for potential drug development were selected for validation by coimmunoprecipitation and colocalization experiments in animal and cellular models.

2020-05-07 | PXD018687 | Pride

The raw data of competitive chemoproteomic experiments for validating the effectiveness of iTarget.

Project description:The raw data for chemoproteomic experiment of 2μM IDRB-Probe-2 with multiple concentrations of competitive IDRB-Binder-2 in Hela. Each concentration includes two measurements taken from distinct samples. 129N and 129C represent the data of 2 μM IDRB-Probe-2. 131N and 131C represent the data of 2 μM IDRB-Probe-2 and 0.2 μM IDRB-Binder-2. 127N and 127C represent the data of 2 μM IDRB-Probe-2 and 2 μM IDRB-Binder-2. 130N and 130C represent the data of 2 μM IDRB-Probe-2 and 6 μM IDRB-Binder-2.

2025-01-06 | PXD058717 | Pride

p53 β-hydroxybutyrylation attenuates p53 activity.

Project description:p53 is an essential tumor suppressor, whose activity is finely tuned by the posttranslational modifications. Previous research has reported that β-hydroxybutyrate (BHB) induces β-hydroxybutyrylation (Kbhb), which is a novel histone posttranslational modification. Here we report that p53 is modified by kbhb and that this modification occurs at lysines 120, 319, and 370 of p53. We demonstrate that the level of p53 kbhb is dramatically increased in cultured cells treated with BHB and in thymus tissues of fasted mice, and that CBP catalyze p53 kbhb. We show that p53 kbhb results in lower levels of p53 acetylation and reduced expression of the p53 downstream genes p21 and PUMA, as well as reduced cell growth arrest and apoptosis in cultured cells under p53-activating conditions. Similar results were observed in mouse thymus tissue under starvation conditions, which result in increased concentrations of serum BHB, and in response to genotoxic stress caused by γ-irradiation to activate p53. Our findings thus show that BHB-mediated p53 kbhb is a novel mechanism of p53 activity regulation, which may explain the link between ketone bodies and tumor, and which may provide promising therapeutic target for cancer treatment.

| S-EPMC6411878 | biostudies-literature

Sarcoptes scabiei Mites Modulate Gene Expression In Human Skin Equivalents

Project description:The purpose of this study was to identify genes in keratinocytes and fibroblasts in human skin equivalents that changed expression in response to the burrowing of live scabies mites. Four biological replicates for the uninfested control condition and five biological replicates for the treatment conditions (live mites, mite extract) were processed for gene expression analysis using Affymetrix Human Gene 1.0 ST arrays.

2013-07-03 | E-GEOD-48459 | biostudies-arrayexpress

Application of Histone Interacting Domains as an Alternative to Antibodies (CIDOP-seq)

Project description:Application of Histone Modification Interacting Domains (HMIDs) as an Alternative to Antibodies. HMIDs and antibodies (taken from ENCODE) were used for precipitation of native chromatin (mononucleosomes) in order to study distinct histone marks. HMIDs used in this study are MPP8 Chromo domains and ATRX ADD (as H3K9me3 binders), Dnmt3a PWWP (as H3K36me3 binder) and CBX7 Chromo (as H3K27me3 binder).

2014-02-06 | E-MTAB-2143 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data