Proteomics

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Identifying Cilary Proteins in Bovine Retinas using a Gentile Extraction Method

ABSTRACT: Mutations in retinal primary cilia are responsible for human blindness but the mechanisms are not fully understood. Characterizing the proteome of an organelle such as cilia, is a fruitful way to understand its function but methods often require considerable sample quantities. Here we develop a method to isolate the primary cilia of photoreceptor cells from bovine retinas. Through LC/MS/MS proteomics analysis we identify proteins enriched for cilia function including ciliopathy disease. This study shows our method can be used to isolate retinal primary cilia to obtain sufficient quantities of native protein samples.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Photoreceptor Cell, Retina

SUBMITTER: Kevin Drew  

LAB HEAD: Kevin Drew

PROVIDER: PXD051952 | Pride | 2024-07-11

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20230207_MSC1158_bovine_retina..comet.pep.xml Pepxml
20230207_MSC1158_bovine_retina.MSGFplus.mzid Mzid
20230207_MSC1158_bovine_retina.mzXML Mzxml
20230207_MSC1158_bovine_retina.pep_count_mFDRpsm001 Other
20230207_MSC1158_bovine_retina.pep_count_mFDRpsm001.log Other
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Publications

Identifying Ciliary Proteins in Mammalian Retinas using a Gentle Extraction Method.

Fredrick Adeline S AS   Claussen Erin R ER   Fischer Samantha N SN   Balasanyants Samson S   Rajaraman Akshaya A   Rosner Andi C AC   Drew Kevin K  

microPublication biology 20240613

Mutations in retinal primary cilia are responsible for human blindness but the mechanisms are not fully understood (Wheway et al., 2014). Characterizing the proteome of an organelle such as cilia, is a fruitful way to understand its function but methods often require considerable sample quantities. Here we develop a method to isolate the primary cilia of photoreceptor cells from bovine retinas. Through LC/MS/MS proteomics analysis we identify proteins enriched for cilia function including ciliop  ...[more]

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