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Mosquito salivary apyrase regulates blood meal hemostasis and drives malaria parasite transmission

ABSTRACT: To identify the putative salivary tPA activator, we fractionated salivary proteins by size-exclusion chromatography and identified fraction Z8 as the strongest tPA activator, whereas the adjacent fractions Z7 and Z9 activated tPA at lower levels. Mass spectrometry analysis of these fractions identified a total of 152 unique proteins.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Anopheles Gambiae (african Malaria Mosquito)

TISSUE(S): Salivary Gland

SUBMITTER: Motoshi Suzuki

LAB HEAD: Joel Vega-Rodriguez

PROVIDER: PXD052155 | Pride | 2025-01-16

REPOSITORIES: pride

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			Action	DRS
	F1-23876.mgf	Mgf
	F1-23876.raw	Raw
	F1-23876_peptides_1_1_0.mzid.gz	Mzid
	F1-23876_protein-peptides.csv	Csv
	F1-23876_proteins.csv	Csv

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Publications

Mosquito salivary apyrase regulates blood meal hemostasis and facilitates malaria parasite transmission.

Pala Zarna Rajeshkumar ZR Alves E Silva Thiago Luiz TL Minai Mahnaz M Crews Benjamin B Patino-Martinez Eduardo E Carmona-Rivera Carmelo C Valenzuela Leon Paola Carolina PC Martin-Martin Ines I Flores-Garcia Yevel Y Cachau Raul E RE Muslinkina Liya L Gittis Apostolos G AG Srivastava Naman N Garboczi David N DN Alves Derron A DA Kaplan Mariana J MJ Fischer Elizabeth E Calvo Eric E Vega-Rodriguez Joel J

Nature communications 20240918 1

The evolution of hematophagy involves a series of adaptations that allow blood-feeding insects to access and consume blood efficiently while managing and circumventing the host's hemostatic and immune responses. Mosquito, and other insects, utilize salivary proteins to regulate these responses at the bite site during and after blood feeding. We investigated the function of Anopheles gambiae salivary apyrase (AgApyrase) in regulating hemostasis in the mosquito blood meal and in Plasmodium transmi ...[more]

PMID: 39294191

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