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Influence of Albumin Concentration on Surface Characteristics and Cellular Responses in the Pre-Incubation of Multi-Walled Carbon Nanotubes

ABSTRACT: Due to the characteristics of multi-walled carbon nanotubes (MWCNTs) to aggregate and bind non-specifically, attempts have been made to inhibit their aggregation and increase their dispersion stability for biomedical applications by coating with bovine serum albumin (BSA). However, BSA concentration has not been studied in detail in terms of cellular response. To investigate the surface proteins formed in 10% fetal bovine serum cell culture media depending on the BSA concentration used to stabilize MWCNTs and to correlate the cellular responses with BSA concentration, we performed nanoflow liquid chromatography–electrospray ionization–tandem mass spectrometry analysis to quantify proteins of the protein corona (PC) and of A549 cells. Results showed that increasing the concentration of pre-incubated BSA changed the composition ratio of the proteins of the PCs adsorbed on the MWCNTs and also decreased the cellular uptake of A549 cells treated with BSA-coated MWCNTs. In the proteomic analysis, proteins related to the ribosome, ribosome biogenesis in eukaryotes, and oxidative phosphorylation pathways were downregulated in the low concentration BSA-treated cell groups, while proteins related to the mRNA surveillance pathway were downregulated in the high-BSA-concentration cell groups. Moreover, circular dichroism and Fourier-transform infrared spectroscopy results showed that the α-helix structure, a secondary structure of the adsorbed proteins, increased as a function of BSA concentration. Taken together, BSA concentration affects the adsorption and secondary structure of PCs on MWCNTs, which in turns affect their intracellular behavior. Our results may provide insight for the design of modification methods to increase the biocompatibility of MWCNTs.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human) Bos Taurus (bovine)

TISSUE(S): Hepatocyte

DISEASE(S): Disease Free

SUBMITTER: Sun Young Lee

LAB HEAD: Jin Gyeong Son

PROVIDER: PXD052226 | Pride | 2024-10-21

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CELL_MWCNT_00BSA_1.tdf	Other
	CELL_MWCNT_00BSA_1.tdf_bin	Other
	CELL_MWCNT_00BSA_2.tdf	Other
	CELL_MWCNT_00BSA_2.tdf_bin	Other
	CELL_MWCNT_00BSA_3.tdf	Other

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Influence of Albumin Concentration on Surface Characteristics and Cellular Responses in the Pre-Incubation of Multi-Walled Carbon Nanotubes

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