Dataset Information

Human gingival fibroblasts infected with HSV-1 UHPLC-MS/MS

ABSTRACT: We conducted a quantitative proteomic analysis of HGFs from early to late HSV-1 infection stage using DIA-based liquid chromatography-tandem mass spectrometry (LC-MS/MS). This work aimed to identify host factors variations and provide information on the various cellular and molecular pathways activated in HGFs. These results help to understand pathological process post HSV-1 infection in periodontal tissues and their potential role in pathogenic mechanisms.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast Of Gingiva, Oral Mucosa

DISEASE(S): Herpes Simplex

SUBMITTER: Kalam Lo

LAB HEAD: Yu Zhang

PROVIDER: PXD053087 | Pride | 2024-11-27

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	0H_Slot2-45_1_4608.d.zip	Other
	0H_Slot2-45_1_4609.d.zip	Other
	0H_Slot2-45_1_4610.d.zip	Other
	12H_Slot2-46_1_4611.d.zip	Other
	12H_Slot2-46_1_4612.d.zip	Other

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Project description:Severe hyposalivation often results from Sjögren’s syndrome or radiation therapy for head and neck cancer. Devastating consequences of hyposalivation, such as rampant dental caries and persistent oral candidiasis, compromise the quality of life in those patients. Clinical management for dry mouth typically involves simple palliative methods or secretagogues, which are designed to stimulate saliva secretion from residual salivary acinar cells present in the glands. However, direct interventions in chronic dryness have yet to be employed in the clinical setting. Despite numerous studies on salivary gland regeneration, the molecular basis governing stem cell transdifferentiation into salivary epithelial precursors (SEP) is largely unknown. Our previously published study clearly indicated mouse bone marrow-derived mesenchymal stem cells (BM-MSCs) can be differentiated into SEP in vitro when co-cultured with isolated primary salivary gland cells without cell-to-cell contact. Our current study utilized iTRAQ-LS-MS/MS-based quantitative proteomics to profile key regulatory factors involved in mouse MSC-to-SEP conversion. We identified 280 differentially expressed proteins in BM-MSCs over the course of 7 days of co-culture. Interestingly, protein expression of salivary transcription factors (STFs), such as transcription factor E2a (TCF3), high mobility group protein 20B (HMG20b), and ankyrin repeat domain-containing protein 56 (ANKRD56) were increased in a time-dependent manner. Notably, pancreas specific transcription factor 1a (PTF1α), muscle, intestine and stomach expression-1 (MIST-1) and achaete-scute complex homolog 3(ASCL3) were newly induced over time in differentiated MSCs. We also identified the expression of Ptf1α in the mouse salivary glands for the first time and verified its expression in independent batches of co-cultured MSCs by western blotting. Furthermore, simulation of the molecular network involving the identified STFs has demonstrated evidence for their perspective roles in salivary gland development during glandular maturation. Thus, our study provides the first extensive proteomic profile of MSC-to-SEP transdifferentiation and identifies novel STFs that may be critical for this process.

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Human gingival fibroblasts infected with HSV-1 UHPLC-MS/MS

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