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Human OTX2 TurboID Analysis in Transfected HDMB03 Cells

ABSTRACT: HDMB03 cells were transiently transfected with plasmids expressing NLS-TurboID or 3xHA-TurboID-OTX2 under the control of a strong CAG promoter. After a 15-minute pulse with 500 µM exogenous biotin, RIPA lysates were prepared and processed with streptavin-sepharose to capture biotinylated proteins that were identified by using mass spectrometry.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Brain Cancer

SUBMITTER: Brad Doble

LAB HEAD: Tamra Erin Ogilvie

PROVIDER: PXD052687 | Pride | 2024-05-31

REPOSITORIES: Pride

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			Action	DRS
	11152021_Tamra_TurboID_A2-OTX2-Oct4_850ng_90min.msf	Msf
	11152021_Tamra_TurboID_A2-OTX2-Oct4_850ng_90min.raw	Raw
	11152021_Tamra_TurboID_C2-OTX2-Oct22_850ng_90min.msf	Msf
	11152021_Tamra_TurboID_C2-OTX2-Oct22_850ng_90min.raw	Raw
	12222021_Tamra_TurboID_Control-1_850ng_90min.msf	Msf

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