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Zbtb48 is a regulator of Mtfp1 expression in zebrafish

ABSTRACT: ZBTB48 (also known as TZAP) is a transcription factor that has previously been reported to bind to telomeres and act as a negative regulator of telomere length in human cell lines. To explore whether transcription factor activity and telomere length regulation are conserved at the organismal level in vertebrates, we generated a zbtb48-/- zebrafish line via CRISPR‒Cas genome editing. The zbtb48-/- mutants displayed no obvious physical or behavioral abnormalities in the first two generations. We found no statistically significant changes in telomere length in first-generation adults. However, for the gene regulatory aspect of Zbtb48, similar to that in human cancer cell lines, we observed downregulation of mtfp1 at both the mRNA and protein levels in the zbtb48-/- mutants. This suggests that mtfp1 is an evolutionarily conserved regulatory target of Zbtb48. Further investigation of the spatiotemporal expression of zbtb48 in previously published zebrafish data revealed low transcript expression in diverse tissues, except in germline stem cells and gametocytes of the gonads. Notably, Mtfp1 protein downregulation was detected in the ovaries of 40 dpf zbtb48-/- mutants and in the testes of both 40 dpf and 10.5-month-old zbtb48-/- mutants.

INSTRUMENT(S): timsTOF HT, Orbitrap Exploris 480

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

SUBMITTER: F Butter

LAB HEAD: Falk Butter

PROVIDER: PXD052763 | Pride | 2025-01-09

REPOSITORIES: pride

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			Action	DRS
	1b_BRF41teloPD.zip	Other
	20191223_SFB1_FBU_FB_Carisa_dr_cont_1.raw	Raw
	20191223_SFB1_FBU_FB_Carisa_dr_cont_2.raw	Raw
	20191223_SFB1_FBU_FB_Carisa_dr_cont_3.raw	Raw
	20191223_SFB1_FBU_FB_Carisa_dr_cont_4.raw	Raw

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Zbtb48 is a regulator of Mtfp1 expression in zebrafish

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