Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human - hMSC Extracellular vesicles produced by starvation (2D) VS in cross-slot chip (3D)

ABSTRACT: Comparison between extracellular vesicles produced from 2D-cultured human Mesenchymal Stem Cells (hMSC) in starvation, and extracellular vesicles produced from spheroids of hMSC hydrodynamically stimulated in a cross-slot millifluidic chip.

INSTRUMENT(S): Orbitrap Astral

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bone Marrow

SUBMITTER: Vanessa Masson

LAB HEAD: Damarys Loew

PROVIDER: PXD054065 | Pride | 2024-10-04

REPOSITORIES: pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Claire_Wilhelm_quanti_2D_chip_Report.sne	Other
	Design_Pride.xlsx	Xlsx
	Z0552LD.raw	Raw
	Z0554LD.raw	Raw
	Z0557LD.raw	Raw

Items per page:

1 - 5 of 13

Similar Datasets

Human - plasma extracellular vesicles from pSS patients, SLE patients and HD

Project description:Proteomic analysis of plasma extracellular vesicles from primary Sjögren syndrom patients, compared to systemic lupus erythematosus patients and helathy donors

2023-06-13 | PXD041452 | Pride

Proteomic analysis of patient-derived xenograft samples from children with B-cell acute lymphoblastic leukemia harboring TCF3::PBX1 and TCF3::HLF translocations

Project description:Despite improved 5-year overall survival rates in B-cell acute lymphoblastic leukemia (B-ALL) due to therapy escalation, effective treatments for relapsed and treatment-resistant disease, especially in specific subtypes like those with TCF3 (formerly E2A) fusions, remain scarce. TCF3, a key regulator of B-cell development, is implicated in various chromosomal translocations linked to lymphoid malignancies, such as TCF3::PBX1 fusion (5% of pediatric B-ALL) and TCF3::HLF fusion (~0.5% of pediatric B-ALL). Current omics research predominantly relies on transcriptomics, but it's increasingly recognized that this may not adequately reflect protein expression, the main targets of drugs and functional entities in biological processes. This study comprehensively analyzed proteomic landscapes of TCF3::HLF+ (n=6) and TCF3::PBX1+ (n=5) B-ALL using primary patient-derived xenografts (PDX), liquid chromatography tandem mass spectrometry, and data-dependent acquisition.

2024-07-05 | PXD053677 | Pride

Extracellular vesicles from fibroblasts stimulated by FGF2

Project description:We compared proteomic analysis of extracellular vesicles (EVs) secreted from human dermal fibroblasts, either control or stimulated by FGF2 and after immunoprecipitation with FGF2-coated beads to isolated FGF2-positive EVs.

2022-12-20 | PXD033141 | Pride

Involvement of the microenvironment in the VC-Resist glioblastoma cells

Project description:In order to understand which molecular mechanisms are involved in radiation resistance and tumor propagation of glioblastoma, our laboratory has developed in-vitro models capable of mimicking the perivascular niche. Indeed, our laboratory hypothesizes that this niche is essential for glioblastoma cells to acquire a radioresistant character. That is why we are considering culturing our glioblastoma cells (GL261 cells) with the factors secreted by the perivascular niche (conditioned medium of mouse brain blood vessels) in order to study which phosphoproteins are activated in the presence of these factors. In parallel, a RNA sequencing analysis was carried out

2024-01-23 | PXD042606 | Pride

Mouse tumor cells secrete heterogenous particles including EVs, ENPs and VLPs into the extracellular medium - a proteome study

Project description:In addition to soluble proteins, cells secrete various types of membrane-enclosed extracellular vesicles (EVs: exosomes, ectosomes and others) and non-vesicular nanoparticles (ENPs) that are thought to play a role in intercellular communication. We exhaustively characterized the protein composition of secreted EVs and ENPs of murine tumor cell lines, including thorough separation of small EVs from co-isolated virus-like particles.

2023-11-27 | PXD039715 | Pride

Human - Cells and Extracellular vesicles of Hela and MNT-1 WT vs CD63 KO Exosomes

Project description:CD63 is a tetraspanin, which organize on cholesterol enriched microdomains, especially on late endosomes. Previous work of our team has shown that CD63 could regulate the sorting of cargoes into ILVs through an ESCRT-independent manner in melanocytes. However, CD63 has also been involved in other trafficking events in other cell type and so far, we have no clear idea of the general process that CD63 would control and would unify these disparate reports. In order to elucidate to general role of CD63 we compared two cell lines, Hela and MNT-1 that we Knock-out for CD63 by CRISPR in both cell lines and investigated the EV generation and the expression level of proteins involved in this process in cell lysates and the EV pellet obtained by SEC.

2024-04-19 | PXD037149 | Pride

GFP-CYYR1 interactome vs. GFP in HEK293 cells

Project description:Cell lysates from HEK293 cells individually transfected with GFP or GFP-CYYR1 were purified on GFP-Trap affinity resin and analyzed by quantitative label-free mass spectrometry. Experiments were performed in 5 biological replicates for each condition.

2024-07-25 | PXD050895 | Pride

Fibroblast-tracks are topographical cues that direct cancer cell migration

Project description:Fibroblasts rely on adhesive structures to migrate. It is generally thought that adhesive structures disassemble once at the rear of the cell to release the cell and allow further movement. However, a significant portion of adhesions is not disassembled and is instead left on the substrate. We observed that virtually all the non-resorbed adhesions remain connected to the substrate by the means of integrin β5. Forward cell migration results in plasma membrane pulling giving rise to tubular structures that are then left on the ground, forming a network of lipid tracks that persist for several days, thus altering substrate’s topography. Cancer cells of different origin exploit fibroblast-tracks as migration railways. The adhesion of cancer cells along tracks is not mediated by focal adhesions, but rather by frustrated clathrin-coated structures (CCSs). Analysis of the composition of track generated by cancer associated fibroblasts allowed to identify integrin αv as the receptor on cancer cells responsible for track recognition. Some cancer cell lines are not able to perform durotaxis (rigidity driven migration), while fibroblasts are very good at it. We thus tested whether, in the presence of fibroblast-tracks, non-durotactic cancer cells would become durotactic by following tracks and this was indeed the case. Hence, fibroblast-tracks are a novel structure capable of physically directing cell migration of different cell types.

2023-09-22 | PXD034091 | Pride

PolQ peptide 4S-P (1482-P/86-P/88-P/93-P) binding partners identification

Project description:We characterized residus of PolQ which are phosphorylated during mitotis. We aimed to identify the partners of the phosphorylated residus of PolQ.

2023-09-18 | PXD042167 | Pride

Extracellular matrix derived from oral mesenchymal stem cells

Project description:To investigate the different signatures of ECM proteins on GSCs and DPSCs as well as the different signatures of ECM proteins during osteogenic differentiation

2024-05-24 | PXD040575 | Pride