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Comparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence

ABSTRACT: Opportunistic infections of the respiratory tract often succeed under a weakened immune response caused by an underlying illness or hospitalization. The human fungal pathogen, Cryptococcus neoformans, and the bacterial pathogen, Klebsiella pneumoniae, are both well-characterized microbes that cause severe infections within immunocompromised individuals. In this study, we simulate a concentration-dependent pulmonary coinfection of a bacterial and fungal pathogen, and profile the proteomic changes by DDA vs. DIA. Dual perspective profiling provides new insights into host defense regulation of infection and pathogenic mechanisms of invasion.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Cryptococcus Neoformans Var. Grubii Serotype A (strain H99 / Atcc 208821 / Cbs 10515 / Fgsc 9487) (filobasidiella Neoformans Var. Grubii) Klebsiella Pneumoniae Subsp. Pneumoniae Cip 52.145 = B5055 Mus Musculus (mouse)

SUBMITTER: Jonathan Krieger

LAB HEAD: Jennifer Geddes-McAlister

PROVIDER: PXD054527 | Pride | 2024-08-13

REPOSITORIES: Pride

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