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Novel RNA-Targeted Proteomics Approach Leads to the Identification of a Key Role for YBX1 in HCMV Replication

ABSTRACT: Understanding the intricate interplay between host factors and viral pathogens remains a focal point of research in molecular virology. Here, we describe a new role for Y-box binding protein 1 (YBX1) during Human Cytomegalovirus (HCMV) infection in regulating the translation of mRNAs encoding the immediate early proteins IE1 and IE2. In this study, we introduce a novel technique for investigating RNA-protein interactions, leveraging the strengths of CRISPR-Cas13, allowing for precise and sensitive exploration of RNA-protein interactions during infection in a temporal manner. Through this approach, we explored the role of YBX1 in HCMV replication, providing a deeper understanding of the molecular host-pathogen interactions. This research not only advances our knowledge of YBX1’s role in viral pathogenesis, but also introduces a powerful tool for unraveling RNA-protein dynamics that can be used in a wide range of biological processes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Fibroblast

DISEASE(S): Disease Free

SUBMITTER: Erik Lenarcic

LAB HEAD: Nathaniel Moorman

PROVIDER: PXD054962 | Pride | 2025-02-13

REPOSITORIES: pride

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			Action	DRS
	20220427_PHCOQEHF_PC965-Moorman_1.raw	Raw
	20220427_PHCOQEHF_PC965-Moorman_10.raw	Raw
	20220427_PHCOQEHF_PC965-Moorman_11.raw	Raw
	20220427_PHCOQEHF_PC965-Moorman_12.raw	Raw
	20220427_PHCOQEHF_PC965-Moorman_2.raw	Raw

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